Divergent Immunological Responses Following Glutaraldehyde Exposure

Details

• Personal Author:
  Azadi S ; Butterworth LF ; Meade BJ
• Description:
  Although Glutaraldehyde (Glut) has been demonstrated to be a moderate contact sensitizer, numerous cases of Occupational asthma related to Glut exposure have been reported. The purpose of these Studies was to examine the close-response relationship between Glut exposure and the development of T cell-mediated vs. IgE- mediated responses. Initial evaluation of the sensitization potential was conducted using the local lymph node assay (LLNA) at concentrations ranging from 0.75% to 2.5%. A concentration-dependent increase in lymphocyte proliferation was observed with EC3 values of 0.072% and 0.089% in CBA and BALB/c mice, respectively. The mouse ear swelling test (MEST) was used to evaluate the potential for Glut to elicit IgE (1/2 h post challenge) and contact hypersensitivity (24 and 48 h post challenge) responses. An immediate response was observed in animals induced and challened with 2.5% Glut, whereas animals induced with 0.1% or 0.75% and challenged with 2.5% exhibited a delayed response 48 h post challenge. IgE-inclucing potential was evaluated by phenotypic analysis of draining lymph node cells and measurement of total serum IgE levels. Only the 2.5% exposed group demonstrated a significant increase (P < 0.01) in the percentage of IgE(+)B220(+) cells and serum IgE. Following 3 days of dermal exposure, a significant increase in IL-4 mRNA in the draining lymph nodes was observed only in the 2.5% exposed group. These results indicate that the development of an immediate vs. a delayed hypersensitivity response following dermal exposure to Glut is at least in part mediated by the exposure concentration. [Description provided by NIOSH]
• Subjects:
  Animals Asthma Asthma, Occupational Immune System Laboratories Occupational Health Respiratory Tract Diseases Safety Skin
• Keywords:
  Animal-studies Bronchial-asthma Dose-response Immune-reaction Laboratory-animals Lymph-nodes Pulmonary-system-disorders Respiratory-system-disorders Sensitivity-testing Skin-exposure
• ISSN:
  0041-008X
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  OD - Office of the Director ; HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  1-8
• Volume:
  197
• Issue:
  1
• NIOSHTIC Number:
  nn:20031579
• Citation:
  Toxicol Appl Pharmacol 2004 May; 197(1):1-8
• Contact Point Address:
  BJ Meade, NIOSH, M-S L1119,1095 Willowdale Rd, Morgantown, WV 26505
• Email:
  sazadi@cdc.gov
• CAS Registry Number:
  Glutaraldehyde (CAS RN 111-30-8)
• Federal Fiscal Year:
  2004
• NORA Priority Area:
  Disease and Injury: Asthma and Chronic Obstructive Pulmonary Disease
• Peer Reviewed:
  True
• Source Full Name:
  Toxicology and Applied Pharmacology
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:767b513682569b64ddeb6c883312304ac8ae199d7051df9d0cdb59ea5b2278843d77a1c4853d2f85d13cd73c2fede1fa25d9fda0767dfc8f5343c3e31fd1b84d
• Download URL:
  https://stacks.cdc.gov/view/cdc/185828/cdc_185828_DS1.pdf
• File Type:
  [PDF - 294.99 KB ]