U.S. flag An official website of the United States government.
Official websites use .gov

A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS

A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

i

The correlation between DNA adducts and chromosomal aberrations in the target organ of benzidine exposed, partially-henatectomized mice.



Details

  • Personal Author:
  • Description:
    The correlation between DNA adducts and chromosomal aberrations was studied in the liver of benzidine (92875) exposed male ICR-Sprague- Dawley-mice after partial hepatectomy. Mice underwent partial hepatectomy, treatment with benzidine (0, 7.8, 19.5, 38.2, or 97.8mg/kg, intraperitoneally), and subcutaneous implantation of a 50 milligram 5-bromodeoxyuridine tablet at 58 hours. Intraperitoneal injection of colcemid (0.04 milligrams per 10 grams body weight) was administered at 70 hours. At 72 hours, mice were killed by cervical dislocation and the livers were removed and subjected to adduct analysis and cytogenic study. No chromosome type aberrations were observed. The majority of damage detected was observed as chromatid breaks. The results demonstrated a clastogenic effect for benzidine in-vivo. Relative adduct labeling (RAL) values were determined and a clear dose response relationship, linear for the dose range tested, was observed. Correlation between chromosomal aberration rate and net RAL was determined. The correlation coefficient for individual mice was 0.41 with a considerable scatter of individual points. Four outlying cases from the set contributed significantly to the variability of data. When the four points were removed, the correlation coefficient was 0.74. The authors conclude that benzidine/DNA adducts are associated with chromosomal aberrations in- vivo, corroborating the findings on associations between in-vitro mutagenesis and transformation and levels of particular carcinogen DNA adducts. Results indicated that DNA adduct levels may be important predictive factors in genotoxicity, including neoplasia. [Description provided by NIOSH]
  • Subjects:
  • Keywords:
  • ISSN:
    0143-3334
  • Document Type:
    Text
  • Funding:
    Grant
  • Genre:
    Journal Article
  • Place as Subject:
  • CIO:
    National Institute for Occupational Safety and Health (NIOSH)
  • Topic:
    Workplace Safety & Health
  • Location:
    North America
  • Volume:
    8
  • Issue:
    12
  • NIOSHTIC Number:
    nn:00175982
  • Citation:
    Carcinogenesis 1987 Dec; 8(12):1899-1905
  • Contact Point Address:
    Prev Med and Community Health University of Texas 301 University Boulevard Galveston, TX 77550
  • CAS Registry Number:
    4,4'-Diaminodiphenyl (CAS RN 92-87-5)
  • Federal Fiscal Year:
    1988
  • Performing Organization:
    University of Texas Medical Branch, Galveston, Texas
  • Peer Reviewed:
    True
  • Start Date:
    19860501
  • Source Full Name:
    Carcinogenesis
  • End Date:
    19861231
  • Collection(s):
  • Main Document Checksum:
    urn:sha-512:80bf985068998cff6f22b2a2f735245b8bd65393915b26d412aff43ae9dd8140b7f7dda8cb4a01be4fb3c0e1ccf935e4eaaa76b10d5d7086ddde6c42a624551b
  • Download URL:
  • File Type:
    Filetype[PDF - 1.28 MB ]
PREVIOUS
ON THIS PAGE
Details
CDC STACKS serves as an archival repository of CDC-published products including scientific findings, journal articles, guidelines, recommendations, or other public health information authored or co-authored by CDC or funded partners.

As a repository, CDC STACKS retains documents in their original published format to ensure public access to scientific information.
BACK TO TOP