Comparative toxicity of halothane, isoflurane, hypoxia, and phenobarbital induction in monolayer cultures of rat hepatocytes

Details

• Personal Author:
  Costa AK ; Heffel DF ; Schieble TM ; Trudell JR
• Description:
  The comparative toxicities of halothane (151677), isoflurane (26675467), hypoxia and phenobarbital induction for monolayer cultures of rat hepatocytes were determined. Adult male Fisher-344- rats were pretreated with phenobarbital by intraperitoneal injections at 80mg/kg/day for four consecutive days. Hepatocytes were isolated from the livers of both treated and nontreated rats and exposed to 1.5 percent halothane or 2.0 percent isoflurane in 1, 2, or 4 percent oxygen for 2 hours. Immediately after exposure or after 6 hours of incubation, supernatants were analyzed for aspartate-aminotransferase release, and Trypan-Blue exclusion was used to determine cells death. The results obtained immediately and at 6 hours after exposure demonstrated that low oxygen levels, halothane, and phenobarbital pretreatment were each highly significant factors in relation to cell death. A toxic effect of isoflurane was not noted under these conditions. The results of this study clearly indicate that the origin of cell death in hepatocyte monolayers was multifactorial with hypoxia, phenobarbital induction and halothane exposure each contributing to the hepatocyte damage observed in this model. [Description provided by NIOSH]
• Subjects:
  Anesthesia Digestive System Digestive System Diseases Laboratories Liver Occupational Health Safety
• Keywords:
  Anesthetics Grants-other Hepatotoxicity In-vitro-studies Laboratory-animals Liver-cells Liver-damage NIOSH-Grant NIOSH-Publication
• ISSN:
  0003-3022
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  California ; OSHA Region 9
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  485-494
• Volume:
  68
• Issue:
  4
• NIOSHTIC Number:
  nn:00187001
• Citation:
  Anesthesiology 1988 Apr; 68(4):485-494
• Contact Point Address:
  Anesthesia Stanford University Department of Anesthesia Stanford, Calif 94305
• CAS Registry Number:
  (±)-Halothane (CAS RN 151-67-7) ; Isoflurane (CAS RN 266Trifluoromethane (CAS RN 75-46-7))
• Federal Fiscal Year:
  1988
• NORA Priority Area:
  Other Occupational Concerns ; Grants Other
• Performing Organization:
  Stanford University, Stanford, California
• Peer Reviewed:
  True
• Start Date:
  19800901
• Source Full Name:
  Anesthesiology
• End Date:
  19901130
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:f373bf1c00e1166e7eb6e677f039d34e854907d126a9e99dbe7f5c12cf18f280a62d7a5ba3ffef539c588de6f7734ca6d3614cb783f1d11269d0c6f9bb4d44e0
• Download URL:
  https://stacks.cdc.gov/view/cdc/204368/cdc_204368_DS1.pdf
• File Type:
  [PDF - 739.98 KB ]