Validation of the Salmonella (SV50)/arabinose-resistant forward mutation assay system with 26 compounds.

Details

• Personal Author:
  Ong T ; Whong Z ; Xu J
• Description:
  The mutagenic sensitivity of the arabinoside resistant (Ara-r) assay using tester (SV-50) was described for a series of 26 compounds known to be mutagenic in the Ames assay or mutagenic only in other systems. Ten of these 26 gave negative responses in the Ara-r and Ames assays both in the plate incorporation test and preincubation test. Preincubation tests enhanced the sensitivity of the Ara-r assay to 13 of 15 compounds by 2.1 to 154 times. The authors conclude that in general the mutagenic response of the Ara-r assay to the 26 compounds tested is similar to that of the Ames assay. It appeared that the preincubation test, which is a simplified combination of the suspension test and the plate incorporation test, might be a better test protocol for the Ara-r assay system with (SV- 50). The study also indicated that the Ara-r forward mutation assay using strain (SV-50) was useful for screening mutagenicity of compounds with different modes of action. The authors conclude that the Ara-r forward mutation assay using (SV-50) is a useful assay system for screening mutagenicities of compounds with different modes of action. [Description provided by NIOSH]
• Subjects:
  Carcinogens Environmental Exposure Environmental Pollution Mutagens Occupational Health Safety
• Keywords:
  Bacteria Biological-monitoring Carcinogenesis Environmental-contamination Environmental-pollution NIOSH-Author
• ISSN:
  0027-5107
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; OSHA Region 4 ; South Carolina ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DRDS - Division of Respiratory Disease Studies
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  79-86
• Volume:
  130
• Issue:
  2
• NIOSHTIC Number:
  nn:00175522
• Citation:
  Mutat Res 1984 Apr; 130(2):79-86
• Federal Fiscal Year:
  1984
• Peer Reviewed:
  True
• Source Full Name:
  Mutation Research
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:cc3c7fcd23c135110fe70930872a9455ddeb250292f6b6ad8de2b981a838dcbdb0df4d26e5069639df50e4eb5978d13945aeeeeba9b4206839027962fdfa4e42
• Download URL:
  https://stacks.cdc.gov/view/cdc/185379/cdc_185379_DS1.pdf
• File Type:
  [PDF - 517.12 KB ]