In vivo and in vitro effects of lead on vascular reactivity in rats.

Details

• Personal Author:
  Vander AJ ; Victery W ; Webb RC ; Winquist RJ
• Description:
  Hypertension caused by increased vascular response to lead (7439921) was investigated in Wistar-rats. Rats were given drinking water containing 100 parts per million (ppm) lead as lead-acetate or sodium-acetate for 7 months. After sacrifice, tail arteries were excised and mounted in a holder connected to a force transducer. Strips of tail artery were electrically stimulated by two platinum wire electrodes parallel to the preparations. Arteries of untreated rats were equilibrated in physiologic salt solution containing either lead-acetate or sodium-acetate for 15 minutes before the addition of vasoactive agents or transmural nerve stimulation. Drugs used were norepinephrine-bitartrate (51401), methoxamine- hydrochloride (61165), and D-600 (16662478), a methoxy derivative of verapamil. Systolic blood pressures of rats treated with lead- acetate were significantly higher than those treated with sodium- acetate. Contractile responses to methoxamine or norepinephrine were significantly greater in tail artery strips treated with lead- acetate. Treatment of arterial strips with D-600 before addition of methoxamine similarly decreased the magnitude of contractile response in lead treated and sodium treated rats. Maximal contractile responses to electrical stimulation in tail artery strips from lead treated rats were significantly greater than in sodium treated rats. In-vitro experiments showed no effects on contractile responses to exogenous norepinephrine or electrical stimulation in sodium-acetate. The authors conclude that the experiments suggest a mechanism whereby chronic exposure to moderate lead concentrations could cause hypertension. [Description provided by NIOSH]
• Subjects:
  Animals Blood Cardiovascular Diseases Cardiovascular System Drinking Water Lead Lead Poisoning Occupational Exposure Occupational Health Safety
• Keywords:
  Animal-studies Author Keywords: Norepinephrine Biological-effects Blood-pressure Cardiovascular-system Cardiovascular-system-disease Chronic-exposure D 600 Drinking-water In-vivo-study Lead-poisoning Methoxamine NIOSH-Grant NIOSH-Publication Tail Artery Vascular Smooth Muscle

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• ISSN:
  1040-0605
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Michigan ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  241
• Issue:
  2
• NIOSHTIC Number:
  nn:00135852
• Citation:
  Am J Physiol Lung Cell Mol Physiol 1981 Aug; 241(2):H211-H216
• Contact Point Address:
  R. Clinton Webb, Physiology University of Michigan 6811 Medical Science II Ann Arbor, Mich
• CAS Registry Number:
  (-)-Norepinephrine (+)-bitartrate (CAS RN 51-40-1) ; (±)-Gallopamil (CAS RN 16662-47-8) ; Lead (CAS RN 7439-92-1) ; Methoxamine hydrochloride (CAS RN 61-16-5)
• Federal Fiscal Year:
  1981
• NORA Priority Area:
  Other Occupational Concerns ; Grants Other
• Performing Organization:
  University of Michigan at Ann Arbor, Ann Arbor, Michigan
• Peer Reviewed:
  True
• Start Date:
  19790901
• Source Full Name:
  American Journal of Physiology: Lung Cellular and Molecular Physiology
• End Date:
  19830831
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:2473696abd350b9199e26b2d1c188a096f38fe1f4902f6b85e5edbb6b3789efc843c3123fb94ed190d1a74c3d8f0828b3a7e3cea55f7ab8b65e71271e3ea74d0
• Download URL:
  https://stacks.cdc.gov/view/cdc/184686/cdc_184686_DS1.pdf
• File Type:
  [PDF - 15.17 MB ]