Disodium tetrachloropalladate (Na2PdCl4), an inhibitor of rat liver mitochondrial electron transport.

Details

• Personal Author:
  Biagini, Raymond E. ; Moorman WJ ; Winston GW
• Description:
  The interaction of disodium-tetrachloropalladate (13820536) (Na2PdCl4) with isolated electron transport and energy metabolism was studied in Sprague-Dawley-rats. Adenosine-triphosphatase (ATPase) activity was assayed by the determination of proton ejection following addition of carbonyl-cyanide-m- chlorophenylhydrazine (555602) (CCCP) or Na2PdCl4. Phosphate were performed after the addition of 400 nanomoles of adenosine- stimulate proton ejection. At the highest concentrations, 64 micromolar, state 3 respiration rates with both succinate and mixture of 5 millimolar (mM) glutamate, 1mM malate, 1mM malonate were inhibited about twenty and forty percent of control values, respectively. Na2PdCl4 demonstrated no ability to stimulate state 4 respiration of ATPase. Along with an inhibition of the electron transport chain, Na2PdCl4 suppressed the rate of accumulation of protons during ADP phosphorylation. The authors conclude that Na2PdCl4 is a relatively potent inhibitor of mitochondrial electron transport and there is a multiphasic inhibitor interaction with the rat liver mitrochondrial electron transport system which yields primary inhibitors of respiration without observable interaction with the coupling system mechanics. [Description provided by NIOSH]
• Subjects:
  Biological Assay Chemistry Chemistry, Analytic Laboratories Occupational Health Safety
• Keywords:
  Analytical-chemistry Analytical-instruments Analytical-methods Bioassays Chemical-analysis Laboratory-techniques NIOSH-Author
• ISSN:
  0378-4274
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  New York ; Ohio ; OSHA Region 2 ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DBBS - Division of Biomedical and Behavioral Sciences
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  165-170
• Volume:
  12
• Issue:
  2
• NIOSHTIC Number:
  nn:00126864
• Citation:
  Toxicol Lett 1982 Jul; 12(2-3):165-170
• CAS Registry Number:
  Carbonyl cyanide (m-chlorophenyl)hydrazone (CAS RN 555-60-2) ; Disodium tetrachloropalladate (CAS RN 13820-53-6)
• Federal Fiscal Year:
  1982
• Peer Reviewed:
  True
• Source Full Name:
  Toxicology Letters
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:17585a8ccb66b9ac96a523c03e3bfe1e715de691360dbf8729c88976ca5bdbabed6e3d078e82a27a67e8791e5a4edcd88a75b0273637000c0da0b4afa1983973
• Download URL:
  https://stacks.cdc.gov/view/cdc/184593/cdc_184593_DS1.pdf
• File Type:
  [PDF - 395.44 KB ]