Metabolism and distribution of two 14C-benzidine-congener-based dyes in rats as determined by GC, HPLC, and radioassays.

Details

• Personal Author:
  Billedeau SM ; Bowman MC ; Nony CR ; Oller WL ; Rowland KL
• Description:
  The absorption, distribution and metabolism of the azo dyes Direct- Red-2 (992596) and Direct-Blue-15 (2429745) were studied. Male Fischer-344-rats were given single doses of 2 milligrams of dye. Urine was collected at intervals and assayed for metabolites radiochemically and by high peformance liquid chromatography. Peak excretion occurred after 8 hours with 6.6 percent of the Direct-Blue- 15 found in urine and 44 percent in the feces. Direct-Red-2 after 8 hours was 8.3 percent in the urine and 35 percent in feces. 3,3- dimethylbenzidine (119937) accumulated in tissue as follows: from Direct-Blue-15 (in microgram equivalents) kidney, 1.14; liver, 4.1; lung, 0.67; stomach, 0.588. From Direct-Red-2 microgram equivalents were 2.9, 8.6, 6.9, and 2.9 for kidney, liver, lung, and stomach, respectively. The authors conclude that both dyes significantly accumulate in the liver, a target organ for rats dosed with benzidine and the site of tumors in rats dosed with benzidine based azo dyes. [Description provided by NIOSH]
• Subjects:
  Chemistry Chemistry, Analytic Chromatography Laboratories Occupational Health Safety
• Keywords:
  Analytical-chemistry Analytical-instruments Analytical-methods Chemical-analysis Chromatographic-analysis Gas-chromatography Laboratory-animals Laboratory-techniques NIOSH-Author Radiochemical-analysis
• ISSN:
  0146-4760
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  Arkansas ; Ohio ; OSHA Region 5 ; OSHA Region 6
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DBBS - Division of Biomedical and Behavioral Sciences
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  164-174
• Volume:
  6
• Issue:
  4
• NIOSHTIC Number:
  nn:00126632
• Citation:
  J Anal Toxicol 1982 Jul-Aug; 6(4):164-174
• CAS Registry Number:
  Direct Blue 15 (CAS RN 2429-74-5) ; o-Tolidine (CAS RN 119-93-7) ; Rirect Red 2 (CAS RN 992-59-6)
• Federal Fiscal Year:
  1982
• Peer Reviewed:
  True
• Source Full Name:
  Journal of Analytical Toxicology
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:9c5c037bb353c9dcab56c50a071403f3d90f439f92147306d83163a0de6924146d10ba8e815c2da1ccb4574603f796e6822964087be2bd125df215c610128793
• Download URL:
  https://stacks.cdc.gov/view/cdc/184587/cdc_184587_DS1.pdf
• File Type:
  [PDF - 2.05 MB ]