Toxicity of ultraviolet-irradiated halothane in mice.

Details

• Personal Author:
  Karis JH ; Menzel DB ; O'Neal FO ; Sabransky M ; Wells CP
• Description:
  The toxicity of halothane (151677) was investigated. Male CD1-mice were exposed for 90 minutes to an atmosphere containing 1.3 percent halothane irradiated by ultraviolet (UV) light or to nonirradiated halothane. After 24 hours, half the mice received 50 milligrams per kilogram of sodium-pentobarbital (57330) and loss of righting reflex and sleeping time were recorded. Liver microsomes were prepared. Protein and enzyme assays were conducted. Additional mice were exposed to 1 percent irradiated or nonirradiated halothane and injected 24 hours later with carbon-14 (C-14) labeled sodium- pentobarbital. Urine and feces were collected and metabolites determined. Sleeping time for mice receiving irradiated halothane increased from 14.3 to 72.5 minutes. Mixed function oxidase activity increased. Only a small amount of C-14 was excreted after 24 hours in mice exposed to UV treated halothane. Serum glutamic oxalacetic transaminase and serum glutamic pyruvic transaminase were 233 and 377 percent of control values, respectively. Hepatic cytochrome P-450 and microsomal aminopyrine demethylase activities decreased in mice exposed to UV irradiated halothane. The authors conclude that debromination and formation of 2,2,2-trifluroacetyl- chloride (354325) are responsible for the increased toxicity of UV irradiated halothane. [Description provided by NIOSH]
• Subjects:
  Chemistry Chemistry, Analytic Enzymes Laboratories Liver Lung Diseases Occupational Health Safety Toxicology
• Keywords:
  Analytical-chemistry Analytical-methods Comparative-toxicology Enzyme-activity Hepatotoxicity Laboratory-animals Lung-disorders NIOSH-Grant NIOSH-Publication
• ISSN:
  0300-483X
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  North Carolina ; OSHA Region 4
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  223-234
• Volume:
  23
• Issue:
  2
• NIOSHTIC Number:
  nn:00126550
• Citation:
  Toxicology 1982 Feb; 23(2-3):223-234
• Contact Point Address:
  Anesthesiology Box 3094 Duke University Durham, N C 27710
• CAS Registry Number:
  (±)-Halothane (CAS RN 151-67-7) ; Pentobarbital sodium (CAS RN 57-33-0) ; Trifluoroacetyl chloride (CAS RN 354-32-5)
• Federal Fiscal Year:
  1982
• Performing Organization:
  Duke University, Durham, North Carolina
• Peer Reviewed:
  True
• Start Date:
  19780901
• Source Full Name:
  Toxicology
• End Date:
  19820831
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:e99371329b3add42a6bd347d2908d7851c1549741c02b52bb3a5301b3ecc62b91bf2415a9411fda4b5383bad98d5208c22dab23eca2fcf1c3f357fd14a9f4d9c
• Download URL:
  https://stacks.cdc.gov/view/cdc/184586/cdc_184586_DS1.pdf
• File Type:
  [PDF - 671.20 KB ]