The effects of chlordane, DDT, and 3-methylcholanthrene upon the metabolism and toxicity of diethyl-4-nitrophenyl phosphorothionate (parathion).

Details

• Personal Author:
  Chapman SK ; Leibman KC
• Description:
  The effect of chlordane (57749), DDT (50293), and 3- methylcholanthrene (56495) (MCA) on the enzyme systems of parathion (56382) metabolism and toxicity was studied in Holtzman-rats and CF- 1-mice. DDT and chlordane were given at a dose of 100 milligrams per kilogram (mg/kg) per day for 3 days, while MCA was administered intraperitoneally in a dose equivalent to 1 percent of the body weight. Animals were killed after 24 hours, and liver homogenates were prepared. Metabolites of (diethyl-C-14)parathion were isolated and identified by thin layer chromatography. Diethyl-hydrogen- phosphorothionate (DHP) production from parathion was stimulated 210 percent in DDT treated rats and the production of paraoxon was stimulated 206 percent. Chlordane stimulated DHP and paraoxon 146 and 166 percent, respectively. MCA resulted in a 83 percent stimulation in DHP and a 182 percent stimulation in paraoxon. The addition of DDT to mouse liver fraction resulted in an enhancement of the pathway responsible for DHP production. A pattern of equal stimulation of both pathways in chlordane-treated rats was observed with mice. MCA administration produced a significant 49 percent depression of DHP production. The author concludes that parathion is metabolized by two separate mixed-function oxidase systems. [Description provided by NIOSH]
• Subjects:
  Energy Metabolism Enzymes Insecticides Laboratories Occupational Health Safety Toxicology
• Keywords:
  Comparative-toxicology Enzyme-activity Laboratory-animals Metabolism NIOSH-Grant NIOSH-Publication Pesticides-and-agricultural-chemicals
• ISSN:
  0041-008X
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Florida ; OSHA Region 4
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  18
• Issue:
  4
• NIOSHTIC Number:
  nn:00113090
• Citation:
  Toxicol Appl Pharmacol 1971 Apr; 18(4):977-987
• Contact Point Address:
  Pharmacology and Therapeutics Univ of Florida Coll of Med Dept of Pharma & Therapeutics Gainesville, Fla 32601
• CAS Registry Number:
  1,2,4,5,6,7,8,8-Octachloro-2,3,3a,4,7,7a-hexahydro-4,7-methano-1H-indene (CAS RN 57-74-9) ; 3-Methylcholanthrene (CAS RN 56-49-5) ; DDT (CAS RN 50-29-3) ; Parathion (CAS RN 56-38-2)
• Federal Fiscal Year:
  1971
• Performing Organization:
  University of Florida Gainesville, Gainesville, Florida
• Peer Reviewed:
  True
• Start Date:
  19710101
• Source Full Name:
  Toxicology and Applied Pharmacology
• End Date:
  19841130
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:a71f00e6c7e8301ce41d39adb91172f108f37418405cacd14fcc33fa6df53c8942cce5d0193f7b3b74a3e95354a2e71f90a61e16f670f6d1ffdf15652167dde4
• Download URL:
  https://stacks.cdc.gov/view/cdc/184413/cdc_184413_DS1.pdf
• File Type:
  [PDF - 870.74 KB ]