Thin layer chromatographic determination of aflatoxin in corn dust.

Details

• Personal Author:
  Burg WR ; Diller T ; Shotwell OL
• Description:
  Thin layer chromatographic (TLC) procedures were developed to determine toxins in airborne corn dust samples. Ground corn and airborne dust samples were collected and prepared for TLC analysis. Analysis included chloroform extraction of dust samples, purification of extracts on a silica gel column, and measurement of aflatoxin (1402682) by either one or two dimensional TLC. The solvent for one dimensional TLC was chloroform, acetone and water, while the solvents for two dimensional TLC were ether, methanol, and water for the first direction and chloroform, acetone, and water for the second direction. Alternatively, the two dimensional method used for the first direction chloroform, acetone, and water, and for the second direction toluene (108883) ethyl acetate (141786), and formic acid (64186). The mean aflatoxin-B1 ground corn. The limits of detection was about 9ng per gram for 0.1 gram samples. [Description provided by NIOSH]
• Subjects:
  Chemistry Chemistry, Analytic Chromatography Dust Occupational Health Safety
• Keywords:
  Analytical-chemistry Analytical-methods Analytical-processes Chemical-analysis Chromatographic-analysis Dust-analysis NIOSH-Grant NIOSH-Publication Qualitative-analysis Vegetable-dusts
• ISSN:
  0004-5756
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Ohio ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  64
• Issue:
  5
• NIOSHTIC Number:
  nn:00119685
• Citation:
  J Assoc Off Anal Chem 1981 Sep; 64(5):1060-1063
• Contact Point Address:
  Environmental Health Kettering Laboratory 3223 Eden Avenue Cincinnati, Ohio 45267
• CAS Registry Number:
  Aflatoxins (CAS RN 1402-68-2) ; Ethyl acetate (CAS RN 141-78-6) ; Formic acid (CAS RN 64-18-6) ; Toluene (CAS RN 108-88-3)
• Federal Fiscal Year:
  1981
• Performing Organization:
  University of Cincinnati, Cincinnati, Ohio
• Peer Reviewed:
  True
• Start Date:
  19790301
• Source Full Name:
  Journal of the Association of Official Analytical Chemists
• End Date:
  19850228
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:958fe8bda25fe685078324dbc0814e56154019c860abca40055f7f9578f7c8d6ca7436938f2eb08b61776f4b71bb7136524dd96516ed9434250c5cf70fe15102
• Download URL:
  https://stacks.cdc.gov/view/cdc/184500/cdc_184500_DS1.pdf
• File Type:
  [PDF - 1.94 MB ]