Genotoxic and mutagenic assays of halothane metabolites in Bacillus subtilis and Salmonella typhimurium.

Details

• Personal Author:
  Cohen EN ; Sachdev K ; Simmon VF
• Description:
  The genotoxic and mutagenic effects of halothane (151677) and its metabolites were studied. Standard mutagenicity assays using Salmonella-typhimurium were performed for halothane, 2-chloro-1,1- difluoroethylene (359104), and 2-bromo-1,1-difluoroethylene. Bacillus-subtilis strains H17 and M45 were grown and treated with test compounds. Toxicities and DNA interactions were assessed. Mutagenicity assays were also performed using polymerase deficient Escherichia-coli. Halothane and its metabolites were nonmutagenic in Salmonella, however, conjugates of these compounds were weakly mutagenic. In Bacillus-subtilis, halothane metabolites inhibited growth of the M45 strain which is deficient in DNA repair ability. There was no differential toxicity found in tests for DNA modifying activity in Escherichia-coli. The authors conclude that there is a possible relationship between halothane metabolism and reported toxic effects associated with occupational anesthetic exposures. [Description provided by NIOSH]
• Subjects:
  Anesthesia Biological Assay Bromine Compounds Energy Metabolism Genetics Hydrocarbons Mutagens Occupational Health Safety Toxicology
• Keywords:
  Anesthetics Bioassays Brominated-hydrocarbons Comparative-toxicology Genetic-disorders Metabolism Metabolites Mutagenesis NIOSH-Grant NIOSH-Publication
• ISSN:
  0003-3022
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  California ; OSHA Region 9
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  31-39
• Volume:
  53
• Issue:
  1
• NIOSHTIC Number:
  nn:00115380
• Citation:
  Anesthesiology 1980 Jul; 53(1):31-39
• Contact Point Address:
  Anesthesia Stanford University 300 Pasteur Drive Stanford, Calif 94305
• CAS Registry Number:
  (±)-Halothane (CAS RN 151-67-7) ; Ethene, 2-chloro-1,1-difluoro- (CAS RN 359-10-4)
• Federal Fiscal Year:
  1980
• Performing Organization:
  Stanford University, Stanford, California
• Peer Reviewed:
  True
• Start Date:
  19770601
• Source Full Name:
  Anesthesiology
• End Date:
  19801231
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:e5f5ab1c737fc6abdf7e391a4c992259752b5cf3ccaefc1f99eca4c81c713fed02695d668d7ca8274858183bae59d50bf1de16067f11e3dbdb48b4e7a6c71972
• Download URL:
  https://stacks.cdc.gov/view/cdc/184436/cdc_184436_DS1.pdf
• File Type:
  [PDF - 684.47 KB ]