Effect of N2 on the mutagenic and lethal activities of ICR-170 in Neurospora crassa.

Details

• Personal Author:
  Whong, Wen-Zong
• Description:
  The relative mutagenic and lethal effects of 2-methoxy-6-chloro-9-(3- (ethyl-2-chloroethyl)aminopropylamino)acridine 2HCl (146598) (ICR- 170) were studied in Neurospora (N)-crassa. Conidial suspensions of adenine-3 presumptive base pair addition or deletion mutants and adenine-3 base pair substitution mutants of N-crassa were prebubbled with nitrogen or oxygen before ICR-170 was added, were bubbled with nitrogen or oxygen after ICR-170 treatment, or were incubated with ICR-170 in the presence of nitrogen or oxygen. Samples were removed after treatment, and mutation and killing activities were determined. The percent survival was significantly less when conidia were exposed to nitrogen during the ICR-170 treatment than when they were exposed to oxygen during treatment, but survival and reversion frequencies were the same when oxygen or nitrogen was given before or after ICR-170 exposure. The nitrogen effect was the same in all strains tested. The high killing activity of ICR-170 induced mutation rate under nitrogen was attributed to enhancement of the mutagenic activity of ICR-170 rather than to selective killing. The author concludes that the increased toxicity and mutagenicity of ICR-170 when given with nitrogen is due to inhibition of conidial respiration in an anoxic environment. [Description provided by NIOSH]
• Subjects:
  Biological Assay Genetics Inorganic Chemicals Mutagens Occupational Health Safety Toxicology
• Keywords:
  Bioassays Genetic-disorders Inorganic-chemicals Mutagenesis NIOSH-Author
• ISSN:
  0027-5107
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  Illinois ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  301-312
• Volume:
  60
• Issue:
  3
• NIOSHTIC Number:
  nn:00117331
• Citation:
  Mutat Res 1979 May; 60(3):301-312
• Contact Point Address:
  Wen·Zong Whong, Department of Biological Science, Illinois State University, Normal, IL 61761 (U.S.A.)
• CAS Registry Number:
  1,3-Propanediamine, N1-(2-chloroethyl)-N3-(6-chloro-2-methoxy-9-acridinyl)-N1-ethyl-, hydrochloride (1:2) (CAS RN 146-59-8)
• Federal Fiscal Year:
  1979
• Peer Reviewed:
  True
• Source Full Name:
  Mutation Research
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:033976d423892d1e1a5846dd33e767d236a2ea7fa698fc16218e284e455a40415b6fdb31c831df67ad69b089a17b402bf637f3c0538c1202d59b293c66fddbec
• Download URL:
  https://stacks.cdc.gov/view/cdc/184460/cdc_184460_DS1.pdf
• File Type:
  [PDF - 3.25 MB ]