Usefulness of Published PCR Primers in Detecting Human Rhinovirus Infection

Faux, Cassandra E.; Arden, Katherine E.; Lambert, Stephen B.; Nissen, Michael D.; Nolan, Terry M.; Chang, Anne B.; Sloots, Theo P.; Mackay, Ian M.

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Usefulness of Published PCR Primers in Detecting Human Rhinovirus Infection

Supporting Files Public Domain

Feb 2011
By Faux, Cassandra E. ; Arden, Katherine E. ; Lambert, Stephen B. ; ...

Details

Alternative Title:

Emerg Infect Dis
Personal Author:

Faux, Cassandra E. ; Arden, Katherine E. ; Lambert, Stephen B. ; Nissen, Michael D. ; Nolan, Terry M. ; Chang, Anne B. ; Sloots, Theo P. ; Mackay, Ian M.
Description:

We conducted a preliminary comparison of the relative sensitivity of a cross-section of published human rhinovirus (HRV)-specific PCR primer pairs, varying the oligonucleotides and annealing temperature. None of the pairs could detect all HRVs in 2 panels of genotyped clinical specimens; >1 PCR is required for accurate description of HRV epidemiology.
Subjects:

Comparative Study Diagnosis Dispatch DNA Primers Genotype Humans Picornaviridae Infections Polymerase Chain Reaction Respiratory Infection Rhinovirus RNA, Viral Sensitivity And Specificity Species Specificity Viruses
Source:

Emerg Infect Dis. 17(2):296-298.
Document Type:

Journal Article
Volume:

17
Issue:

2
Collection(s):

Emerging Infectious Diseases
Main Document Checksum:

urn:sha256:e089cca797b2a2b7e21f1a92e77e9948f00ffbd50d92dfb51a56bfec465eeac0
Download URL:

https://stacks.cdc.gov/view/cdc/18435/cdc_18435_DS1.pdf
File Type:

[PDF - 248.15 KB ]

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