An in vivo method to prepare normal Schwann cells free of axons and myelin.

Details

• Personal Author:
  Krygier-Brevart V ; Spencer PS ; Weinberg HJ ; Zabrenetzky V
• Description:
  A technique for preparing a viable population of Schwann cells based on the phenomenon of Wallerian degeneration was demonstrated. The sciatic nerves of albino-rabbits were exposed, crushed, and cut. The nerve stumps were then sewn into adjacent muscle surface to impede regenerative processes. Distal nerve stumps containing the proliferated Schwann cells were removed 5 to 12 weeks later, and morphologically studied by light and electron microscopy. Schwann cells formed longitudinal columns separated by endoneurial connective tissue. The authors conclude that this technique has the following advantages: simplicity and accessibility, large tissue yield, applicability to different species for comparative study, prolonged in-vivo viability and usefulness for analysis of axonal regulation of Schwann cell function and determination of the role of Schwann cell alteration in peripheral nerve reaction. [Description provided by NIOSH]
• Subjects:
  Cytology Histology Laboratories Nervous System Occupational Health Safety
• Keywords:
  Cytological-analysis In-vivo-study Laboratory-animals Membrane-dysfunction Nerve-fibers Nerves-degeneration Neurons Neurotoxic-effects NIOSH-Grant NIOSH-Publication Nuclear-membrane Peripheral-nervous-system Research-laboratories

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• ISSN:
  0006-8993
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  New York ; OSHA Region 2
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  119-226
• Volume:
  165
• Issue:
  1
• NIOSHTIC Number:
  nn:00091807
• Citation:
  Brain Res 1979 Apr; 165(1):119-126
• Contact Point Address:
  Pathology Albert Einstein Coll of Med 1300 Morris Park Avenue Bronx, N Y 10461
• Federal Fiscal Year:
  1979
• NORA Priority Area:
  Neurotoxic Disorders ; Neurotoxic Effects
• Performing Organization:
  Yeshiva University, New York, New York
• Peer Reviewed:
  True
• Start Date:
  19751101
• Source Full Name:
  Brain Research
• End Date:
  19850331
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:313d51c3845b2ac44c62d70550d8112716612b902210d378a20217a79bdd5da20750c431fcc3abf05ddc86d67a479568559cc6d9c9bd00944218f0cc0d7122f9
• Download URL:
  https://stacks.cdc.gov/view/cdc/184281/cdc_184281_DS1.pdf
• File Type:
  [PDF - 473.75 KB ]