Storage concentration stability study of 1- and 2-bromopropane spiked human urine.

Details

• Personal Author:
  B'Hymer C ; Cheever KL
• Description:
  A six week study was performed to verify the concentration stability of 1- and 2-bromopropane (BP) spiked urine samples. Both are volatile solvents believed to be present in urine of exposed workers. 1-Bromopropane (1-BP) is a commonly used industrial solvent, and 2-bromopropane (2-BP) is often found as an impurity component in industrial grade 1-BP. Both are of a health concern for exposed workers because of their chronic toxicity. Central neurological and peripheral neuropathy disorders have been reported in workers exposed to 1-BP; reproductive and hematopoietic disorders have been reported in workers exposed to 2-BP. Human dermal exposure to 1- and 2-BP is of concern by this laboratory, and proper handling and storage of field collected urine samples to preserve these volatile compounds is necessary. Spiked urine stored in glass serum vials with crimped caps and Teflon lined septa at 2-4 degrees-C were compared to spiked urine stored in polypropylene tubes frozen at -80 degrees-C, the traditional urine storage technique at this laboratory. The spiked urine samples were assayed by headspace analysis at two and six week time points. In the headspace procedure used, urine samples were diluted with deionized water and placed into a sealed headspace vial. A static-headspace sampler (Teledyne-Tekmar Model 7000) was used to heat each sample at 75 degrees-C for a 35 minute equilibrium time. Quantification of the two analytes was by means of a gas chromatograph equipped with a dimethylpolysiloxane capillary column and an electron capture detector. 1-Bromobutane was used as an internal standard for this test procedure. The glass serum vials (n=10) demonstrated full recovery of both analytes after six weeks of storage; 1-BP recovery was 113% and 2-BP was 103% for 2 microg/ml level spikes. The plastic tubes (n=10) had recoveries of 68% for 1-BP and 77% for 2-BP after six weeks of storage.
• Subjects:
  Accidents, Occupational Aerospace Medicine Air Pollutants, Occupational Air Pollution Convection Environmental Monitoring Metals Metals, Heavy Occupational Health Preceptorship Safety Welding

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• Keywords:
  Analytical-chemistry Analytical-methods Analytical-processes Chemical-processing Chemical-properties Neurological-diseases Neurological-system Solvents Urinalysis
• Publisher:
  National Institute for Occupational Safety and Health
• Document Type:
  Text
• Genre:
  Abstract
• Place as Subject:
  Ohio ; OSHA Region 3 ; OSHA Region 5 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DART - Division of Applied Research and Technology
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  1
• NIOSHTIC Number:
  nn:20027252
• Citation:
  Occupational and Environmental Exposures of Skin to Chemicals, June 12-15, 2005, Stockholm, Sweden. Morgantown, WV: National Institute for Occupational Safety and Health, 2005 Jun; :1
• CAS Registry Number:
  1-Bromopropane (CAS RN 106-94-5) ; 2-Bromopropane (CAS RN 75-26-3)
• Federal Fiscal Year:
  2005
• NORA Priority Area:
  Research Tools and Approaches: Exposure Assessment Methods
• Peer Reviewed:
  False
• Source Full Name:
  Occupational and Environmental Exposures of Skin to Chemicals, June 12-15, 2005, Stockholm, Sweden
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:44e574636c0c8ed7b4296d23ed4be3d27ee96416d2753f702b3dea1d4ec964da09ae824c659c23cc04b1db1279a210d582fc161666af0a9f4320192ffe70bfd8
• Download URL:
  https://stacks.cdc.gov/view/cdc/181321/cdc_181321_DS1.pdf
• File Type:
  [PDF - 481.87 KB ]