Discriminatory Ability of Hypervariable Variable Number Tandem Repeat Loci in Population-based Analysis of Mycobacterium tuberculosis Strains, London, UK

Details

• Alternative Title:
  Emerg Infect Dis
• Personal Author:
  Velji, Preya ; Nikolayevskyy, Vladyslav ; Brown, Timothy ; Drobniewski, Francis
• Description:
  To address conflicting results about the stability of variable number tandem repeat (VNTR) loci and their value in prospective molecular epidemiology of Mycobacterium tuberculosis, we conducted a large prospective population-based analysis of all M. tuberculosis strains in a metropolitan setting. Optimal and reproducible conditions for reliable PCR and fragment analysis, comprising enzymes, denaturing conditions, and capillary temperature, were identified for a panel of hypervariable loci, including 3232, 2163a, 1982, and 4052. A total of 2,261 individual M. tuberculosis isolates and 265 sets of serial isolates were analyzed by using a standardized 15-loci VNTR panel, then an optimized hypervariable loci panel. The discriminative ability of loci varied substantially; locus VNTR 3232 varied the most, with 19 allelic variants and Hunter-Gaston index value of 0.909. Hypervariable loci should be included in standardized panels because they can provide consistent comparable results at multiple settings, provided the proposed conditions are adhered to.
• Subjects:
  Bacteria Bacterial Genetics Genetic Markers Genotype Genotyping Humans London Metropolitan Area Minisatellite Repeats Molecular Epidemiology Mycobacterium Tuberculosis Reproducibility Research Tuberculosis Tuberculosis, Pulmonary Tuberculosis And Other Mycobacteria

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• Source:
  Emerg Infect Dis. 15(10):1609-1616.
• Document Type:
  Journal Article
• Volume:
  15
• Issue:
  10
• Collection(s):
  Emerging Infectious Diseases
• Main Document Checksum:
  urn:sha256:ee966c4f5d802588272c25b55ea9e6c2284b88ff9c606f50c3e12ca56eb69494
• Download URL:
  https://stacks.cdc.gov/view/cdc/17903/cdc_17903_DS1.pdf
• File Type:
  [PDF - 537.24 KB ]