Performance evaluation of the Streck ARM-D® Kit, β-Lactamase for molecular detection of acquired β-lactamase genes

Details

• Alternative Title:
  J Glob Antimicrob Resist
• Personal Author:
  Yoo, Brian B. ; Yamamoto, Norihisa ; Quintero, Justina Ilutsik ; Machado, Maria Jose ; Sabour, Sarah ; Blosser, Sara ; Karlsson, Maria ; Rasheed, James Kamile ; Brown, Allison C.
• Description:
  Objectives: ; Despite clinical relevance, commercially available molecular tools for accurate β-lactamase detection are limited. In this study, we evaluated the performance of the ARM-D® Kit, β-lactamase, a commercially available multiplex PCR assay designed to detect nine β-lactamase genes, including the five major plasmid-mediated carbapenemases, ESBL and AmpC genes circulating in the United States. ; Methods: ; A diverse collection of 113 Gram-negative isolates, including 42 with multiple β-lactamases genes, was selected from the U.S. Centers for Disease Control and Prevention (CDC) & Food and Drug Administration (FDA) Antimicrobial Resistance Isolate Bank, to represent the most frequently detected bacterial species carrying plasmid-mediated β-lactam resistance genes. ; Results: ; Results were compared with whole genome sequence data. Of 164 β-lactamase gene targets with 49 unique variants, all were detected correctly without any cross-reactivity. The sensitivity and specificity were 100% (164/164) and 99.9% (852/853), respectively. ; Conclusion: ; The ARM-D® Kit, β-lactamase detected a wide range of β-lactamase genotypes at a low upfront cost. The Streck assay represents a suitable, comprehensive tool for the detection of key β-lactamase resistance genes of public health concern in the United States.
• Subjects:
  • beta-Lactamases
  • Humans
  • Multiplex Polymerase Chain Reaction
  • Gram-Negative Bacteria
  • United States
  • Sensitivity and Specificity
  • Reagent Kits, Diagnostic
  • Plasmids
  • beta-Lactam Resistance
  • Bacterial Proteins
  More +
• Keywords:
  • Carbapenemase
  • Molecular detection
  • Extended-spectrum β-lactamase (esbl)
  • AmpC
• Source:
  J Glob Antimicrob Resist. 39:54-58
• Pubmed ID:
  39168374
• Pubmed Central ID:
  PMC11663688
• Document Type:
  Journal Article
• Funding:
  CC999999/ImCDC/Intramural CDC HHSUnited States/
• Volume:
  39
• Download URL:
  https://stacks.cdc.gov/view/cdc/175240/cdc_175240_DS1.pdf
• File Type:
  [PDF - 357.41 KB]
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha-512:c04f58aa04589c694c4fd1c6802d9582990f7f60545ed0696610bc486f33015de1e0ffe4ba3d6c143e84576b596b72726116f49da824242b3ec8390e2a8ed766