Characteristics of 263K Scrapie Agent in Multiple Hamster Species

Details

• Alternative Title:
  Emerg Infect Dis
• Personal Author:
  Meade-White, Kimberly D. ; Barbian, Kent D. ; Race, Brent ; Favara, Cynthia ; Gardner, Don ; Taubner, Lara ; Porcella, Stephen ; Race, Richard
• Description:
  Transmissible spongiform encephalopathy (TSE) diseases are known to cross species barriers, but the pathologic and biochemical changes that occur during transmission are not well understood. To better understand these changes, we infected 6 hamster species with 263K hamster scrapie strain and, after each of 3 successive passages in the new species, analyzed abnormal proteinase K (PK)-resistant prion protein (PrPres) glycoform ratios, PrPres PK sensitivity, incubation periods, and lesion profiles. Unique 263K molecular and biochemical profiles evolved in each of the infected hamster species. Characteristics of 263K in the new hamster species seemed to correlate best with host factors rather than agent strain. Furthermore, 2 polymorphic regions of the prion protein amino acid sequence correlated with profile differences in these TSE-infected hamster species.
• Subjects:
  Amino Acid Sequence Animals Biochemical Profile Cricetinae Endopeptidase K Hamsters Immunohistochemistry Molecular Profile Molecular Sequence Data Prion Diseases Prions PRNP Amino Acid Sequence Prnp Nucleotide Sequence PrPSc Proteins Research Scrapie Sequence Analysis, DNA Serial Passage Species Barriers Species Specificity Transmissible Spongiform Encephalopathy TSE

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• Source:
  Emerg Infect Dis. 15(2):207-215.
• Document Type:
  Journal Article
• Volume:
  15
• Issue:
  2
• Collection(s):
  Emerging Infectious Diseases
• Main Document Checksum:
  urn:sha256:cfb8854b226b8e7f28fdf7feaf7b3e9792aa45305e5e221c9c6b0c6671d9095d
• Download URL:
  https://stacks.cdc.gov/view/cdc/17435/cdc_17435_DS1.pdf
• File Type:
  [PDF - 629.43 KB ]