Details:

Alternative Title:
Emerg Infect Dis
Personal Author:
Hansman, Grant S. ; Oka, Tomoichiro ; Sakon, Naomi ; Takeda, Naokazu
Hansman, Grant S. ; Oka, Tomoichiro ; Sakon, Naomi ; Takeda, Naokazu Less -
Description:
Sapovirus (SaV) is a causative agent of gastroenteritis. On the basis of capsid protein (VP1) nucleotide sequences, SaV can be divided into 5 genogroups (GI-GV), of which the GI, GII, GIV, and GV strains infect humans. SaV is uncultivable, but expression of recombinant VP1 in insect cells results in formation of viruslike particles (VLPs) that are antigenically similar to native SaV. In this study, we newly expressed SaV GII and GIV VLPs to compare genetic and antigenic relationships among all human SaV genogroups. Hyperimmune antiserum samples against VLPs reacted strongly with homologous VLPs. However, several antiserum samples weakly cross-reacted against heterologous VLPs in an antibody ELISA. Conversely, an antigen ELISA showed that VLPs of SaV in all human genogroups were antigenically distinct. These findings indicate a likely correspondence between SaV antigenicity and VP1 genogrouping and genotyping.
Subject:
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Antiserum Caliciviridae Infections Capsid Protein Capsid Proteins Cross-reactivity Gastroenteritis Genogroup Genotype Humans Phylogeny Research Sapovirus Sequence Analysis, RNA Virus-like Particles Genotype
Source:
Emerg Infect Dis. 13(10):1519-1525.
Document Type:
Journal Article
Collection(s):
Emerging Infectious Diseases
Main Document Checksum:
[+]
urn:sha256:168af66d9542eba25ce183e56b548fbba4c64306faa0fe9ef73d4905a7e0d3e2
Download URL:
https://stacks.cdc.gov/view/cdc/16793/cdc_16793_DS1.pdf
File Type:

Supporting Files

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