Enhanced Subtyping Scheme for Salmonella Enteritidis

Zheng, Jie; Keys, Christine E.; Zhao, Shaohua; Meng, Jianghong; Brown, Eric W.

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Enhanced Subtyping Scheme for Salmonella Enteritidis

Supporting Files Public Domain

Dec 2007
By Zheng, Jie ; Keys, Christine E. ; Zhao, Shaohua ; ...

Details

Alternative Title:

Emerg Infect Dis
Personal Author:

Zheng, Jie ; Keys, Christine E. ; Zhao, Shaohua ; Meng, Jianghong ; Brown, Eric W.
Description:

To improve pulsed-field gel electrophoresis-based strain discrimination of 76 Salmonella Enteritidis strains, we evaluated 6 macro-restriction endonucleases, separately and in various combinations. One 3-enzyme subset, SfiI/PacI/NotI, was highly discriminatory. Five different indices, including the Simpson diversity index, supported this 3-enzyme combination for improved differentiation of S. Enteritidis.
Subjects:

Bacterial Typing Techniques Clone Differentiation Dispatch Electrophoresis, Gel, Pulsed-Field Genetic Diversity Molecular Epidemiology Phylogeny Restriction Endonuclease Salmonella Enterica Salmonella Enteritidis Subtyping
Source:

Emerg Infect Dis. 13(12):1932-1935.
Document Type:

Journal Article
Volume:

13
Issue:

12
Collection(s):

Emerging Infectious Diseases
Main Document Checksum:

urn:sha256:cd4c8b5b82e36624cac52c5733c346b9155f64e803a2ce767dc51c8827a8969f
Download URL:

https://stacks.cdc.gov/view/cdc/16725/cdc_16725_DS1.pdf
File Type:

[PDF - 210.55 KB ]

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