Loop-Mediated Isothermal Amplification for Influenza A (H5N1) Virus

Jayawardena, Shanthi; Cheung, Chung Y.; Barr, Ian; Chan, Kwok H.; Chen, Honglin; Guan, Yi; Peiris, J.S. Malik; Poon, Leo L.M.

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Loop-Mediated Isothermal Amplification for Influenza A (H5N1) Virus

Supporting Files Public Domain

Jun 2007
By Jayawardena, Shanthi ; Cheung, Chung Y. ; Barr, Ian ; ...

Details

Alternative Title:

Emerg Infect Dis
Personal Author:

Jayawardena, Shanthi ; Cheung, Chung Y. ; Barr, Ian ; Chan, Kwok H. ; Chen, Honglin ; Guan, Yi ; Peiris, J.S. Malik ; Poon, Leo L.M.
Description:

We describe a 1-step reverse-transcription loop-mediated isothermal amplification assay for detection of highly pathogenic avian influenza A (H5N1) viruses. The assay was tested by using a panel of highly pathogenic H5N1 subtypes isolated over the past 10 years and clinical specimens. The assay produced negative results for all non-H5N1 subtypes.
Subjects:

Animals Avian Influenza Virus Dispatch DNA, Viral H5N1 Humans Influenza, Human Influenza A Virus, H5N1 Subtype Influenza In Birds Loop-mediated Isothermal Amplification Molecular Detection Molecular Diagnostic Techniques Molecular Sequence Data Poultry Virology
Source:

Emerg Infect Dis. 13(6):899-901.
Document Type:

Journal Article
Volume:

13
Issue:

6
Collection(s):

Emerging Infectious Diseases
Main Document Checksum:

urn:sha256:34aa93048eee2d864d8e754600117424e4ab899bb56dca7a7114f74716e8416a
Download URL:

https://stacks.cdc.gov/view/cdc/16658/cdc_16658_DS1.pdf
File Type:

[PDF - 206.63 KB ]

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