Isolation and Characterization of Novel Human Parechovirus from Clinical Samples

Details

• Alternative Title:
  Emerg Infect Dis
• Personal Author:
  Watanabe, Kanako ; Oie, Masayasu ; Higuchi, Masaya ; Nishikawa, Makoto ; Fujii, Masahiro
• Description:
  Using Vero cells, we isolated a virus (NII561-2000) from a cerebrospinal fluid specimen of a 1-year-old girl with Reye syndrome. The determined amino acid sequence of the virus indicated that the isolate was a human parechovirus (HPeV), a member of Picornaviridae. Neutralization test showed that the NII561-2000 virus had distinct antigenicity to HPeV-1, HPeV-2, and HPeV-3, and that the sequence was distinct from these types as well as from HPeV-4 and HPeV-5. Thus, we propose the virus (NII561-2000) as the prototype of HPeV-6. We isolated 10 NII561-2000-related viruses, 14 HPeV-1, 16 HPeV-3, and 1 HPeV-4 of 41 HPeVs from various clinical samples collected in Niigata, Japan. Clinical symptoms of the persons infected with the NII561-2000-related viruses were infectious gastroenteritis, rash, upper respiratory tract infection, and paralysis, in addition to Reye syndrome in the 1-year-old girl.
• Subjects:
  Fatal Outcome Female Humans Infant Molecular Sequence Data Parechovirus Phylogeny Picornaviridae Infections Reye Syndrome
• Source:
  Emerg Infect Dis. 13(6):889-895.
• Document Type:
  Journal Article
• Place as Subject:
  Japan
• Location:
  Asia
• Volume:
  13
• Issue:
  6
• Collection(s):
  Emerging Infectious Diseases
• Main Document Checksum:
  urn:sha256:193b6c5012315a90bcf73269f8a14de49514404bb872d3981757d4d2b0afa074
• Download URL:
  https://stacks.cdc.gov/view/cdc/16473/cdc_16473_DS1.pdf
• File Type:
  [PDF - 251.91 KB ]