Ongoing Genome Reduction in Mycobacterium ulcerans

Rondini, Simona; Käser, Michael; Stinear, Timothy; Tessier, Michel; Mangold, Cyrill; Dernick, Gregor; Naegeli, Martin; Portaels, Françoise; Certa, Ulrich; Pluschke, Gerd

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Ongoing Genome Reduction in Mycobacterium ulcerans

Supporting Files Public Domain

Jul 2007
By Rondini, Simona ; Käser, Michael ; Stinear, Timothy ; ...

Details

Alternative Title:

Emerg Infect Dis
Personal Author:

Rondini, Simona ; Käser, Michael ; Stinear, Timothy ; Tessier, Michel ; Mangold, Cyrill ; Dernick, Gregor ; Naegeli, Martin ; Portaels, Françoise ; Certa, Ulrich ; Pluschke, Gerd
Description:

Elucidation of the transmission, epidemiology, and evolution of Mycobacterium ulcerans, the causative agent of Buruli ulcer, is hampered by the striking lack of genetic diversity of this emerging pathogen. However, by using a prototype plasmid-based microarray that covered 10% of the genome, we found multiple genomic DNA deletions among 30 M. ulcerans clinical isolates of diverse geographic origins. Many of the changes appear to have been mediated by insertion sequence (IS) elements IS2404 and IS2606, which have high copy numbers. Classification of the deleted genes according to their biological functions supports the hypothesis that M. ulcerans has recently evolved from the generalist environmental M. marinum to become a niche-adapted specialist. The substantial genomic diversity, along with a prototype microarray that covered a small portion of the genome, suggests that a genome-wide microarray will make available a genetic fingerprinting method with the high resolution required for microepidemiologic studies.
Subjects:

Adaptation, Physiological Buruli Ulcer Chromosomes, Bacterial Deletional Genomic Diversity DNA Transposable Elements Evolution, Molecular Genetic Variation Genome, Bacterial Humans Microarray Molecular Sequence Data Mycobacterium Marinum Mycobacterium Ulcerans Oligonucleotide Array Sequence Analysis Research Species Specificity Strain Variation Virulence
Source:

Emerg Infect Dis. 13(7):1008-1015.
Document Type:

Journal Article
Volume:

13
Issue:

7
Collection(s):

Emerging Infectious Diseases
Main Document Checksum:

urn:sha256:cccfedcf2aa957a31812f523daf3bf4e0c31e5129c6495675b80a0d1f75895aa
Download URL:

https://stacks.cdc.gov/view/cdc/16243/cdc_16243_DS1.pdf
File Type:

[PDF - 338.07 KB ]

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