Identifying and Quantifying Genotypes in Polyclonal Infections due to Single Species

Details

• Alternative Title:
  Emerg Infect Dis
• Personal Author:
  Colborn, James M. ; Koita, Ousmane A. ; Cissé, Ousmane ; Bagayoko, Mamadou W. ; Guthrie, Edward J. ; Krogstad, Donald J.
• Description:
  Simultaneous infection with multiple pathogens of the same species occurs with HIV, hepatitis C, Epstein-Barr virus, dengue, tuberculosis, and malaria. However, available methods do not distinguish among or quantify pathogen genotypes in individual patients; they also cannot test for novel insertions and deletions in genetically modified organisms. The strategy reported here accomplishes these goals with real-time polymerase chain reaction (PCR) and capillary electrophoresis. Real-time PCR with allotype-specific primers defines the allotypes (strains) present and the intensity of infection (copy number). Capillary electrophoresis defines the number of genotypes within each allotype and the intensity of infection by genotype. This strategy can be used to study the epidemiology of emerging infectious diseases with simultaneous infection by multiple genotypes, as demonstrated here with malaria. It also permits testing for insertions or deletions in genetically modified organisms that may be used for bioterrorism.
• Subjects:
  Adolescent Adult Allotype Animals Capillary Electrophoresis Child Copy Number Deletions Electrophoresis, Capillary Genotype Humans Insertions Malaria, Falciparum PCR Plasmodium Falciparum Polymerase Chain Reaction Research Time Factors

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• Source:
  Emerg Infect Dis. 12(3):475-482.
• Document Type:
  Journal Article
• Volume:
  12
• Issue:
  3
• Collection(s):
  Emerging Infectious Diseases
• Main Document Checksum:
  urn:sha256:7de70cdd305e3f8f3b7d96f5fbbb6117abb286b85b9261930d69ac9a7087d158
• Download URL:
  https://stacks.cdc.gov/view/cdc/15931/cdc_15931_DS1.pdf
• File Type:
  [PDF - 320.52 KB ]