Longitudinally Profiling Neutralizing Antibody Response to SARS Coronavirus with Pseudotypes

Temperton, Nigel J.; Chan, Paul K.; Simmons, Graham; Zambon, Maria C.; Tedder, Richard S.; Takeuchi, Yasuhiro; Weiss, Robin A.

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Longitudinally Profiling Neutralizing Antibody Response to SARS Coronavirus with Pseudotypes

Published Date:

Mar 2005

Source:

Emerg Infect Dis. 11(3):411-416.

[PDF - 203.46 KB]

Details:

Personal Authors:

Temperton, Nigel J. ; Chan, Paul K. ; Simmons, Graham ; Zambon, Maria C. ; Tedder, Richard S. ; ... More ▼

Keywords:

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Description:

The severe acute respiratory syndrome-associated coronavirus (SARS-CoV) spike protein (S) is a major target for neutralizing antibodies. Retroviral SARS-CoV S pseudotypes have been constructed and used to develop an in vitro microneutralization assay that is both sensitive and specific for SARS-CoV neutralizing antibodies. Neutralization titers measured by this assay are highly correlated to those measured by an assay using replication-competent SARS-CoV. No cross-neutralization occurred with human sera known to contain antibodies to coronavirus strains OC43 and 229E. The pseudotype assay was used to profile neutralizing antibody responses against SARS-CoV S in sequential serum samples taken from 41 confirmed SARS patients during the 2003 outbreak in Hong Kong and shows long-lasting immunity in most recovered patients. The pseudotype assay does not require handling live SARS virus; it is a useful tool to determine neutralizing titers during natural infection and the preclinical evaluation of candidate vaccines.

Document Type:

Journal Article

Collection(s):

Emerging Infectious Diseases

Supporting Files:

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