Details:

Alternative Title:
Emerg Infect Dis
Personal Author:
Chua, Paul K.B. ; Corkill, John E. ; Hooi, Poh Sim ; Cheng, Soo Choon ; Winstanley, Craig ; ... More +
Chua, Paul K.B. ; Corkill, John E. ; Hooi, Poh Sim ; Cheng, Soo Choon ; Winstanley, Craig ; Hart, C. Anthony Less -
Description:
An obligate intracellular bacterium was isolated from urine samples from 7 (3.5%) of 202 fruit bats (Eonycteris spelaea) in peninsular Malaysia. The bacterium produced large membrane-bound inclusions in human, simian, and rodent cell lines, including epithelial, fibroblastlike, and lymphoid cells. Thin-section electron microscopy showed reticulate bodies dividing by binary fission and elementary bodies in the inclusions; mitochondria surrounded the inclusions. The inclusions were positive for periodic acid-Schiff stain but could not be stained by fluorescein-labeled anti-Chlamydia trachomatis major outer membrane protein monoclonal antibody. The bacterium was resistant to penicillin and streptomycin (MICs > 256 mg/L) but susceptible to tetracycline (MIC = 0.25 mg/L) and chloramphenicol (MIC = 0.5 mg/L). Sequence analysis of the 16SrRNA gene indicated that it was most closely related to 2 isolates of Waddlia chondrophila (94% and 96% identity). The 16S and 23S rRNA gene signatures were only 91% identical. We propose this novel bacterium be called W. malaysiensis.
Subjects:
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3T3 Cells 16S RDNA Inclusions Acetylmuramyl-Alanyl-Isoglutamine Animals Anti-Bacterial Agents Antimicrobial Susceptibility Base Sequence Cercopithecus Aethiops Chiroptera Chlamydia Chlamydiales Cricetinae DNA, Bacterial DNA, Ribosomal Spacer Fruit Bat Humans Mice Microbial Sensitivity Tests Microscopy, Electron Molecular Sequence Data Phylogeny Polymerase Chain Reaction Research RNA, Ribosomal, 16S RNA, Ribosomal, 23S Sequence Alignment Vero Cells Waddlia
Source:
Emerg Infect Dis. 11(2):271-277.
Document Type:
Journal Article
Collection(s):
Emerging Infectious Diseases
Main Document Checksum:
[+]
urn:sha256:04504f5e4333797bde04e73e414fed801839c63711964180d59e6f6c26ef23c4
Download URL:
https://stacks.cdc.gov/view/cdc/15678/cdc_15678_DS1.pdf
File Type:

Supporting Files

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