Details:

Alternative Title:
Emerg Infect Dis
Personal Author:
Chua, Paul K.B. ; Corkill, John E. ; Hooi, Poh Sim ; Cheng, Soo Choon ; Winstanley, Craig ; ... More +
Chua, Paul K.B. ; Corkill, John E. ; Hooi, Poh Sim ; Cheng, Soo Choon ; Winstanley, Craig ; Hart, C. Anthony ; Less -
Description:
An obligate intracellular bacterium was isolated from urine samples from 7 (3.5%) of 202 fruit bats (Eonycteris spelaea) in peninsular Malaysia. The bacterium produced large membrane-bound inclusions in human, simian, and rodent cell lines, including epithelial, fibroblastlike, and lymphoid cells. Thin-section electron microscopy showed reticulate bodies dividing by binary fission and elementary bodies in the inclusions; mitochondria surrounded the inclusions. The inclusions were positive for periodic acid-Schiff stain but could not be stained by fluorescein-labeled anti-Chlamydia trachomatis major outer membrane protein monoclonal antibody. The bacterium was resistant to penicillin and streptomycin (MICs > 256 mg/L) but susceptible to tetracycline (MIC = 0.25 mg/L) and chloramphenicol (MIC = 0.5 mg/L). Sequence analysis of the 16SrRNA gene indicated that it was most closely related to 2 isolates of Waddlia chondrophila (94% and 96% identity). The 16S and 23S rRNA gene signatures were only 91% identical. We propose this novel bacterium be called W. malaysiensis.
Subject:
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3T3 Cells
16S RDNA Inclusions
Acetylmuramyl-Alanyl-Isoglutamine
Animals
Anti-Bacterial Agents
Antimicrobial Susceptibility
Base Sequence
Cercopithecus Aethiops
Chiroptera
Chlamydia
Chlamydiales
Cricetinae
DNA, Bacterial
DNA, Ribosomal Spacer
Fruit Bat
Humans
Mice
Microbial Sensitivity Tests
Microscopy, Electron
Molecular Sequence Data
Phylogeny
Polymerase Chain Reaction
Research
RNA, Ribosomal, 16S
RNA, Ribosomal, 23S
Sequence Alignment
Vero Cells
Waddlia

Source:
Emerg Infect Dis. 11(2):271-277. ;
Document Type:
Journal Article ;
Collection(s):
Emerging Infectious Diseases
Main Document Checksum:
[+]
urn:sha256:04504f5e4333797bde04e73e414fed801839c63711964180d59e6f6c26ef23c4
File Type:

Supporting Files

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