Details:

Alternative Title:
Emerg Infect Dis
Personal Author:
Sampath, Rangarajan ; Hofstadler, Steven A. ; Blyn, Lawrence B. ; Eshoo, Mark W. ; Hall, Thomas A. ; ... More +
Sampath, Rangarajan ; Hofstadler, Steven A. ; Blyn, Lawrence B. ; Eshoo, Mark W. ; Hall, Thomas A. ; Massire, Christian ; Levene, Harold M. ; Hannis, James C. ; Harrell, Patina M. ; Neuman, Benjamin ; Buchmeier, Michael J. ; Jiang, Yun ; Ranken, Raymond ; Drader, Jared J. ; Samant, Vivek ; Griffey, Richard H. ; McNeil, John A. ; Crooke, Stanley T. ; Ecker, David J. ; Less -
Description:
We describe a new approach for infectious disease surveillance that facilitates rapid identification of known and emerging pathogens. The process uses broad-range polymerase chain reaction (PCR) to amplify nucleic acid targets from large groupings of organisms, electrospray ionization mass spectrometry for accurate mass measurements of PCR products, and base composition signature analysis to identify organisms in a sample. We demonstrate this principle by using 14 isolates of 9 diverse Coronavirus spp., including the severe acute respiratory syndrome-associated coronavirus (SARS-CoV). We show that this method could identify and distinguish between SARS and other known CoV, including the human CoV 229E and OC43, individually and in a mixture of all 3 human viruses. The sensitivity of detection, measured by using titered SARS-CoV spiked into human serum, was approximate, equals1 PFU/mL. This approach, applicable to the surveillance of bacterial, viral, fungal, or protozoal pathogens, is capable of automated analysis of >900 PCR reactions per day.
Subject:
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Animals
Base Sequence
Communicable Diseases, Emerging
Coronaviridae
Coronaviridae Infections
Emerging Pathogens
ESI Mass Spectrometry
Humans
Infectious Disease Surveillance
Molecular Epidemiology
PCR
Polymerase Chain Reaction
Population Surveillance
Research
RNA, Viral
SARS Virus
Sensitivity And Specificity
Spectrometry, Mass, Electrospray Ionization

Source:
Emerg Infect Dis. 11(3):373-379. ;
Document Type:
Journal Article ;
Collection(s):
Emerging Infectious Diseases
Main Document Checksum:
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urn:sha256:9d0540282d80c4af378cb9972318c729aacb2934aaab7ea883c742e57f28e968
File Type:

Supporting Files

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