Details:

Alternative Title:
Emerg Infect Dis
Personal Author:
Bruijnesteijn van Coppenraet, Lesla E.S.; Kuijper, Edward J.; Lindeboom, Jerome A.; Prins, Jan M.; Claas, Eric C. J.;
Bruijnesteijn van Coppenraet, Lesla E.S.; Kuijper, Edward J.; Lindeboom, Jerome A.; Prins, Jan M.; Claas, Eric C. J.; Less -
Description:
Infections associated with Mycobacterium haemophilum are underdiagnosed because specific culture methods required for its recovery are not applied routinely. Using polymerase chain reaction (PCR) technology on fine needle aspirates and biopsied specimens from 89 children with cervicofacial lymphadenitis, we assessed the importance of M. haemophilum. Application of a Mycobacterium genus-specific real-time PCR in combination with amplicon sequencing and a M. haemophilum-specific PCR resulted in the recognition of M. haemophilum as the causative agent in 16 (18%) children with cervicofacial lymphadenitis. M. avium was the most frequently found species (56%), and M. haemophilum was the second most commonly recognized pathogen. Real-time PCR results were superior to culture because only 9 (56%) of the 16 diagnosed M. haemophilum infections were positive by culture.
Subject:
[+]
Biopsy
Biopsy, Needle
Child
Humans
Lymphadenitis
MGB
Minor Groove Binder
Mycobacterium Haemophilum
Mycobacterium Infections
Polymerase Chain Reaction
Real-time PCR
Research
Sensitivity And Specificity
Species Specificity
Lymphadenitis

Source:
Emerg Infect Dis. 11(1):62-68.;
Document Type:
Journal Article;
Collection(s):
Emerging Infectious Diseases
Main Document Checksum:
[+]
urn:sha256:03261a6dd2b4f78dbfa89f18a41b1ebea14ab4ea5b5d5dd1655b17c239cad5f1
File Type:

Supporting Files

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