Details:

Alternative Title:
Emerg Infect Dis
Personal Author:
Bruijnesteijn van Coppenraet, Lesla E.S. ; Kuijper, Edward J. ; Lindeboom, Jerome A. ; Prins, Jan M. ; Claas, Eric C. J.
Bruijnesteijn van Coppenraet, Lesla E.S. ; Kuijper, Edward J. ; Lindeboom, Jerome A. ; Prins, Jan M. ; Claas, Eric C. J. Less -
Description:
Infections associated with Mycobacterium haemophilum are underdiagnosed because specific culture methods required for its recovery are not applied routinely. Using polymerase chain reaction (PCR) technology on fine needle aspirates and biopsied specimens from 89 children with cervicofacial lymphadenitis, we assessed the importance of M. haemophilum. Application of a Mycobacterium genus-specific real-time PCR in combination with amplicon sequencing and a M. haemophilum-specific PCR resulted in the recognition of M. haemophilum as the causative agent in 16 (18%) children with cervicofacial lymphadenitis. M. avium was the most frequently found species (56%), and M. haemophilum was the second most commonly recognized pathogen. Real-time PCR results were superior to culture because only 9 (56%) of the 16 diagnosed M. haemophilum infections were positive by culture.
Subject:
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Biopsy Biopsy, Needle Child Humans Lymphadenitis Mgb Minor Groove Binder Mycobacterium Haemophilum Mycobacterium Infections Polymerase Chain Reaction Real-Time Pcr Research Sensitivity And Specificity Species Specificity
Source:
Emerg Infect Dis. 11(1):62-68.
Document Type:
Journal Article
Collection(s):
Emerging Infectious Diseases
Main Document Checksum:
[+]
urn:sha256:03261a6dd2b4f78dbfa89f18a41b1ebea14ab4ea5b5d5dd1655b17c239cad5f1
Download URL:
https://stacks.cdc.gov/view/cdc/15639/cdc_15639_DS1.pdf
File Type:

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