Genetic polymorphism among Cryptosporidium parvum isolates: evidence of two distinct human transmission cycles.

Details

• Alternative Title:
  Emerg Infect Dis
• Personal Author:
  Peng, M. M. ; Xiao, L. ; Freeman, A. R. ; Arrowood, M. J. ; Escalante, A. A. ; Weltman, A. C. ; Ong, C. S. ; Mac Kenzie, W. R. ; Lal, A. A. ; Beard, C. B.
• Description:
  We report the results of molecular analysis of 39 isolates of Cryptosporidium parvum from human and bovine sources in nine human outbreaks and from bovine sources from a wide geographic distribution. All 39 isolates could be divided into either of two genotypes, on the basis of genetic polymorphism observed at the thrombospondin-related adhesion protein (TRAP-C2) locus. Genotype 1 was observed only in isolates from humans. Genotype 2, however, was seen in calf isolates and in isolates from a subset of human patients who reported direct exposure to infected cattle or consumed items thought to be contaminated with cattle faces. Furthermore, experimental infection studies showed that genotype 2 isolates were infective to mice or calves under routine laboratory conditions, whereas genotype 1 isolates were not. These results support the occurrence of two distinct transmission cycles of C. parvum in humans.
• Subjects:
  Amino Acid Sequence Animals Base Sequence Cattle Cryptosporidium Parvum DNA, Protozoan Genotype Humans Mice Molecular Sequence Data Polymerase Chain Reaction Polymorphism, Genetic

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• Source:
  Emerg Infect Dis. 3(4):567-573.
• Document Type:
  Journal Article
• Volume:
  3
• Issue:
  4
• Collection(s):
  Emerging Infectious Diseases
• Main Document Checksum:
  urn:sha256:78e10bd63948fb6f5730b0b80777c71e61967e8bc95247156189a2c881e6f119
• Download URL:
  https://stacks.cdc.gov/view/cdc/15443/cdc_15443_DS1.pdf
• File Type:
  [PDF - 232.73 KB ]