Sensitivity and specificity for malaria classification of febrile persons by rapid diagnostic test, microscopy, parasite DNA, histidine-rich protein 2, and IgG: Dakar, Senegal 2015

Details

• Alternative Title:
  Int J Infect Dis
• Personal Author:
  Badiane, Aida ; Thwing, Julie ; Williamson, John ; Rogier, Eric ; Diallo, Mamadou Alpha ; Ndiaye, Daouda
• Description:
  Objectives:
  
  Different methods detecting Plasmodium parasite infection or exposure are available, but systematic comparison of all these methodologies to predict malaria infection is lacking. Understanding the characteristics of respective tests is helpful to choose the most appropriate tests for epidemiological or research purposes.
  
  Methods:
  
  Microscopy, RDT, and PCR was performed for 496 patients presenting with febrile illness in Dakar, Senegal in 2015. Blood samples had laboratory multiplex assays performed for IgG serology and detection of HRP2 antigen. Sensitivity (Se) and specificity (Sp) for different tests were calculated using PCR as the gold standard for detecting active infection. Modeling through latent class analysis (LCA) compared each test to a modeled gold standard for Se/Sp estimates.
  
  Results:
  
  Against PCR, Se/Sp were 95.2%/93.7% for RDT, 90.4%/100.0% for microscopy, and 97.9%/48.1% for lab HRP2 detection. Compared to the modeled gold standard, Se of microscopy was 93.5%, and Se of RDT, PCR, and lab HRP2 detection were all greater than 99%. Se/Sp of IgG serology were substantially for detecting active infection.
  
  Conclusions:
  
  Compared to single tests, a combinatorial LCA approach of multiple biomarkers for detecting malaria infection from patient samples provides greater sensitivity and specificity for epidemiological estimates and research objectives.
  
  
• Subjects:
  Animals Antigens, Protozoan Diagnostic Tests, Routine DNA Fever Histidine Humans Immunoglobulin G Malaria Malaria, Falciparum Microscopy Parasites Plasmodium Falciparum Protozoan Proteins Senegal Sensitivity And Specificity

  More +
• Keywords:
  Antigen Detection Diagnostics IgG Detection Latent Class Analysis Malaria PCR
• Source:
  Int J Infect Dis. 121:92-97
• Pubmed ID:
  35504551
• Pubmed Central ID:
  PMC10948003
• Document Type:
  Journal Article
• Funding:
  CC999999/ImCDC/Intramural CDC HHSUnited States/
• Volume:
  121
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:19944bd391f6f9dfe789b1788114b83957e2305fd6540d6f89c9aa29ba1a105a
• Download URL:
  https://stacks.cdc.gov/view/cdc/151762/cdc_151762_DS1.pdf
• File Type:
  [PDF - 206.95 KB ]