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LincRNA-Cox2 functions to regulate inflammation in alveolar macrophages during acute lung injury
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4 15 2022
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Source: J Immunol. 208(8):1886-1900
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Alternative Title:J Immunol
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Personal Author:
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Description:Our respiratory system is vital to protect us from the surrounding nonsterile environment; therefore, it is critical for a state of homeostasis to be maintained through a balance of inflammatory cues. Recent studies have shown that actively transcribed noncoding regions of the genome are emerging as key regulators of biological processes, including inflammation. | is one such example of an inflammatory inducible long intergenic noncoding RNA functioning to fine-tune immune gene expression. Using bulk and single-cell RNA sequencing, in addition to FACS, we find that | is most highly expressed in the lung and is most upregulated after LPS-induced lung injury (acute lung injury [ALI]) within alveolar macrophages, where it functions to regulate inflammation. We previously reported that | functions to regulate its neighboring protein Ptgs2 in |, and in this study, we use genetic mouse models to confirm its role in regulating gene expression more broadly in | during ALI. Il6, Ccl3, and Ccl5 are dysregulated in the |-deficient mice and can be rescued to wild type levels by crossing the deficient mice with our newly generated | transgenic mice, confirming that this gene functions in | Many genes are specifically regulated by | within alveolar macrophages originating from the bone marrow because the phenotype can be reversed by transplantation of wild type bone marrow into the |-deficient mice. In conclusion, we show that | is a |-acting long noncoding RNA that functions to regulate immune responses and maintain homeostasis within the lung at baseline and on LPS-induced ALI.
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Pubmed ID:35365562
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Pubmed Central ID:PMC9038212
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Volume:208
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Issue:8
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[PDF-2.56 MB]
