Nested polymerase chain reaction for amplification of the Cryptosporidium oocyst wall protein gene.

Pedraza-Díaz, S.; Amar, C.; Nichols, G. L.; McLauchlin, J.

i

Nested polymerase chain reaction for amplification of the Cryptosporidium oocyst wall protein gene.

Supporting Files Public Domain

2001 Jan-Feb
By Pedraza-Díaz, S. ; Amar, C. ; Nichols, G. L. ; ...

File Language:

English

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Alternative Title:

Emerg Infect Dis
Personal Author:

Pedraza-Díaz, S. ; Amar, C. ; Nichols, G. L. ; McLauchlin, J.
Description:

We developed a sensitive nested polymerase chain reaction procedure for the Cryptosporidium oocyst wall protein (COWP) gene. Amplification and genotyping were successful in 95.2% of 1,680 fecal samples, 77.6% by the unnested and 17.6% by the nested COWP procedure. The COWP gene was amplified from 2,128 fecal samples: 71 from livestock animals and 2,057 from humans. This series included 706 cases from seven drinking water-associated outbreaks and 51 cases from five swimming pool-associated outbreaks, as well as 1,300 sporadic cases.
Subjects:

Animals Cryptosporidium Genotype Polymerase Chain Reaction Protozoan Proteins Rna, Ribosomal, 18S Water Water Supply
Source:

Emerg Infect Dis. 7(1):49-56.
Document Type:

Journal Article
Volume:

7
Issue:

1
Collection(s):

Emerging Infectious Diseases
Main Document Checksum:

urn:sha256:dfb743ba126e06b5d074b9d4a876f663c4e46c4531bfad4cb84d94699d1433f1
Download URL:

https://stacks.cdc.gov/view/cdc/15062/cdc_15062_DS1.pdf
File Type:

[PDF - 62.94 KB ]

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File Language:

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