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Nested polymerase chain reaction for amplification of the Cryptosporidium oocyst wall protein gene.
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2001 Jan-Feb
Source: Emerg Infect Dis. 7(1):49-56.
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Alternative Title:Emerg Infect Dis
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Description:We developed a sensitive nested polymerase chain reaction procedure for the Cryptosporidium oocyst wall protein (COWP) gene. Amplification and genotyping were successful in 95.2% of 1,680 fecal samples, 77.6% by the unnested and 17.6% by the nested COWP procedure. The COWP gene was amplified from 2,128 fecal samples: 71 from livestock animals and 2,057 from humans. This series included 706 cases from seven drinking water-associated outbreaks and 51 cases from five swimming pool-associated outbreaks, as well as 1,300 sporadic cases.
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