Details:

Alternative Title:
Emerg Infect Dis
Personal Author:
Fredricks, D. N. ; Jolley, J. A. ; Lepp, P. W. ; Kosek, J. C. ; Relman, D. A. ;
Fredricks, D. N. ; Jolley, J. A. ; Lepp, P. W. ; Kosek, J. C. ; Relman, D. A. ; Less -
Description:
Rhinosporidium seeberi, a microorganism that can infect the mucosal surfaces of humans and animals, has been classified as a fungus on the basis of morphologic and histochemical characteristics. Using consensus polymerase chain reaction (PCR), we amplified a portion of the R. seeberi 18S rRNA gene directly from infected tissue. Analysis of the aligned sequence and inference of phylogenetic relationships showed that R. seeberi is a protist from a novel clade of parasites that infect fish and amphibians. Fluorescence in situ hybridization and R. seeberi- specific PCR showed that this unique 18S rRNA sequence is also present in other tissues infected with R. seeberi. Our data support the R. seeberi phylogeny recently suggested by another group. R. seeberi is not a classic fungus, but rather the first known human pathogen from the DRIPs clade, a novel clade of aquatic protistan parasites (Ichthyosporea).
Subject:
[+]
Animals
Cloning, Molecular
Dog Diseases
Dogs
Eukaryota
Genes, RRNA
Humans
In Situ Hybridization, Fluorescence
Microscopy, Electron
Molecular Sequence Data
Phylogeny
Polymerase Chain Reaction
Rhinosporidiosis
Rhinosporidium
RNA, Ribosomal, 18S
Sequence Analysis, DNA

Source:
Emerg Infect Dis. 6(3):273-282.
Document Type:
Journal Article ;
Collection(s):
Emerging Infectious Diseases
Main Document Checksum:
[+]
urn:sha256:036c58ff2eb0674633715f18814c4168cdf04e44a415087176d12b0c3bc2d387
File Type:

Supporting Files

• 	10827117.nxml	txt
  	license.txt	txt

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