Detection of Anaplasma phagocytophilum DNA in Ixodes Ticks (Acari: Ixodidae) from Madeira Island and Setúbal District, Mainland Portugal

Details

• Alternative Title:
  Emerg Infect Dis
• Personal Author:
  Santos, Ana Sofia ; Santos-Silva, Maria Margarida ; Almeida, Victor Carlos ; Bacellar, Fátima ; Dumler, John Stephen ; Santos, Ana Sofia ; Santos-Silva, Maria Margarida ; Almeida, Victor Carlos ; Bacellar, Fátima ; Dumler, John Stephen
• Description:
  A total of 278 Ixodes ticks, collected from Madeira Island and Setubal District, mainland Portugal, were examined by polymerase chain reaction (PCR) for the presence of Anaplasma phagocytophilum. Six (4%) of 142 Ixodes ricinus nymphs collected in Madeira Island and 1 nymph and 1 male (2%) of 93 I. ventalloi collected in Setubal District tested positive for A. phagocytophilum msp2 genes or rrs. Infection was not detected among 43 I. ricinus on mainland Portugal. All PCR products were confirmed by nucleotide sequencing to be identical or to be most closely related to A. phagocytophilum. To our knowledge, this is the first evidence of A. phagocytophilum in ticks from Setubal District, mainland Portugal, and the first documentation of Anaplasma infection in I. ventalloi. Moreover, these findings confirm the persistence of A. phagocytophilum in Madeira Island's I. ricinus.
• Subjects:
  Animals Arachnid Vectors DNA, Bacterial Ixodes Phylogeny
• Source:
  Emerg Infect Dis. 10(9):1643-1648. ; Emerg Infect Dis. 10(9):1643-1648.
• Document Type:
  Journal Article
• Place as Subject:
  Portugal
• Volume:
  10
• Issue:
  9
• Collection(s):
  Emerging Infectious Diseases
• Main Document Checksum:
  urn:sha256:efcf04fb0ba2e37590977eb9f34de05fafee39231aba5488de8df29962c722a0
• Download URL:
  https://stacks.cdc.gov/view/cdc/14638/cdc_14638_DS1.pdf
• File Type:
  [PDF - 203.07 KB ]