Details:

Alternative Title:
Emerg Infect Dis
Publisher's site:
http://dx.doi.org/10.3201/eid1005.030875
Personal Author:
Xu, Dongping ; Zhang, Zheng ; Chu, Fuliang ; Li, Yonggang ; Jin, Lei ; ... More +
Xu, Dongping ; Zhang, Zheng ; Chu, Fuliang ; Li, Yonggang ; Jin, Lei ; Zhang, Lingxia ; Gao, George F. ; Wang, Fu-Sheng Less -
Description:
To characterize genetic variation of severe acute respiratory syndrome-associated coronavirus (SARS-CoV) transmitted in the Beijing area during the epidemic outbreak of 2003, we sequenced 29 full-length S genes of SARS-CoV from 20 hospitalized SARS patients on our unit, the Beijing 302 Hospital. Viral RNA templates for the S-gene amplification were directly extracted from raw clinical samples, including plasma, throat swab, sputum, and stool, during the course of the epidemic in the Beijing area. We used a TA-cloning assay with direct analysis of nested reverse transcription-polymerase chain reaction products in sequence. One hundred thirteen sequence variations with nine recurrent variant sites were identified in analyzed S-gene sequences compared with the BJ01 strain of SARS-CoV. Among them, eight variant sites were, we think, the first documented. Our findings demonstrate the coexistence of S-gene sequences with and without substitutions (compared to BJ01) in samples analyzed from some patients.
Subject:
[+]
Adult
Aged
Communicable Diseases, Emerging
Disease Outbreaks
Female
Genetic Variation
Hospitalization
Humans
Male
Membrane Glycoproteins
Middle Aged
Research
Reverse Transcriptase Polymerase Chain Reaction
RNA, Viral
SARS Virus
Sequence Analysis, DNA
Severe Acute Respiratory Syndrome
Spike Glycoprotein, Coronavirus
Viral Envelope Proteins

Source:
Emerg Infect Dis. 10(5):789-794.
Document Type:
Journal Article
Place as Subject:
China
Collection(s):
Emerging Infectious Diseases
Main Document Checksum:
[+]
urn:sha256:9dd9ce48c50fadc980fc04f2b55730ace73fa615b8e68923a952b821b88de4a3
File Type:

Supporting Files

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