Details:

Alternative Title:
Emerg Infect Dis
Publisher's site:
http://dx.doi.org/10.3201/eid1003.030683
Personal Author:
Vicenzi, Elisa ; Canducci, Filippo ; Pinna, Debora ; Mancini, Nicasio ; Carletti, Silvia ; ... More +
Vicenzi, Elisa ; Canducci, Filippo ; Pinna, Debora ; Mancini, Nicasio ; Carletti, Silvia ; Lazzarin, Adriano ; Bordignon, Claudio ; Poli, Guido ; Clementi, Massimo Less -
Description:
During the recent severe acute respiratory (SARS) outbreak, the etiologic agent was identified as a new coronavirus (CoV). We have isolated a SARS-associated CoV (SARS-CoV) strain by injecting Vero cells with a sputum specimen from an Italian patient affected by a severe pneumonia; the patient traveled from Vietnam to Italy in March 2003. Ultrastructural analysis of infected Vero cells showed the virions within cell vesicles and around the cell membrane. The full-length viral genome sequence was similar to those derived from the Hong-Kong Hotel M isolate. By using both real-time reverse transcription-polymerase chain reaction TaqMan assay and an infectivity plaque assay, we determined that approximately 360 viral genomes were required to generate a PFU. In addition, heparin (100 microg/mL) inhibited infection of Vero cells by 50%. Overall, the molecular and biologic characteristics of the strain HSR1 provide evidence that SARS-CoV forms a fourth genetic coronavirus group with distinct genomic and biologic features.
Subjects:
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Animals Cercopithecus Aethiops Communicable Diseases, Emerging Genome, Viral Humans Isolation Male Plaque Assay Real-time PCR Research Reverse Transcriptase Polymerase Chain Reaction SARS-coronavirus SARS Virus Sequence Severe Acute Respiratory Syndrome Sputum Vero Cells
Source:
Emerg Infect Dis. 10(3):413-418.
Document Type:
Journal Article
Collection(s):
Emerging Infectious Diseases
Main Document Checksum:
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urn:sha256:64ed8d338c1f5c80bb3e25e4b5dd513b60fc93cdbd03f9b644a21b26381ca5fe
Download URL:
https://stacks.cdc.gov/view/cdc/14479/cdc_14479_DS1.pdf
File Type:

Supporting Files

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