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Real-Time Reverse Transcription–Polymerase Chain Reaction Assay for SARS-associated Coronavirus
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February 2004
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Source: Emerging Infectious Diseases. 10(2):311-316.
[PDF-429.06 KB]
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Alternative Title:Emerging Infectious Diseases
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Description:A real-time reverse transcription-polymerase chain reaction (RT-PCR) assay was developed to rapidly detect the severe acute respiratory syndrome-associated coronavirus (SARS-CoV). The assay, based on multiple primer and probe sets located in different regions of the SARS-CoV genome, could discriminate SARS-CoV from other human and animal coronaviruses with a potential detection limit of <10 genomic copies per reaction. The real-time RT-PCR assay was more sensitive than a conventional RT-PCR assay or culture isolation and proved suitable to detect SARS-CoV in clinical specimens. Application of this assay will aid in diagnosing SARS-CoV infection.
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