Real-Time Reverse Transcription–Polymerase Chain Reaction Assay for SARS-associated Coronavirus

Details

• Alternative Title:
  Emerging Infectious Diseases
• Personal Author:
  Emery, Shannon L. ; Erdman, Dean D. ; Bowen, Michael D. ; Newton, Bruce R. ; Winchell, Jonas M. ; Meyer, Richard F. ; Tong, Suxiang ; Cook, Byron T. ; Holloway, Brian P. ; McCaustland, Karen A. ; Rota, Paul A. ; Bankamp, Bettina ; Lowe, Luis E. ; Ksiazek, Tom G. ; Bellini, William J. ; Anderson, Larry J.
• Description:
  A real-time reverse transcription-polymerase chain reaction (RT-PCR) assay was developed to rapidly detect the severe acute respiratory syndrome-associated coronavirus (SARS-CoV). The assay, based on multiple primer and probe sets located in different regions of the SARS-CoV genome, could discriminate SARS-CoV from other human and animal coronaviruses with a potential detection limit of <10 genomic copies per reaction. The real-time RT-PCR assay was more sensitive than a conventional RT-PCR assay or culture isolation and proved suitable to detect SARS-CoV in clinical specimens. Application of this assay will aid in diagnosing SARS-CoV infection.
• Subjects:
  Animals Base Sequence Cercopithecus Aethiops DNA, Viral DNA Primers Humans Reproducibility Of Results Research Reverse Transcriptase Polymerase Chain Reaction RNA, Viral SARS Virus Sensitivity And Specificity Severe Acute Respiratory Syndrome Vero Cells

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• Source:
  Emerging Infectious Diseases. 10(2):311-316.
• Document Type:
  Journal Article
• Collection(s):
  Emerging Infectious Diseases
• Main Document Checksum:
  urn:sha256:7732610bf717b40fe72294ddd4b9054504742888d55123f1cc63af0d70d07406
• Download URL:
  https://stacks.cdc.gov/view/cdc/7753/cdc_7753_DS1.pdf
• File Type:
  [PDF - 429.06 KB ]