Susceptibility of Pigs and Chickens to SARS Coronavirus

Details

• Alternative Title:
  Emerg Infect Dis
• Personal Author:
  Weingartl, Hana M. ; Copps, John ; Drebot, Michael A. ; Marszal, Peter ; Smith, Greg ; Gren, Jason ; Andonova, Maya ; Pasick, John ; Kitching, Paul ; Czub, Markus
• Description:
  An outbreak of severe acute respiratory syndrome (SARS) in humans, associated with a new coronavirus, was reported in Southeast Asia, Europe, and North America in early 2003. To address speculations that the virus originated in domesticated animals, or that domestic species were susceptible to the virus, we inoculated 6-week-old pigs and chickens intravenously, intranasally, ocularly, and orally with 106 PFU of SARS-associated coronavirus (SARS-CoV). Clinical signs did not develop in any animal, nor were gross pathologic changes evident on postmortem examinations. Attempts at virus isolation were unsuccessful; however, viral RNA was detected by reverse transcriptase-polymerase chain reaction in blood of both species during the first week after inoculation, and in chicken organs at 2 weeks after inoculation. Virus-neutralizing antibodies developed in the pigs. Our results indicate that these animals do not play a role as amplifying hosts for SARS-CoV.
• Subjects:
  Animals Antibodies, Viral Base Sequence Cell Line Cells, Cultured Cercopithecus Aethiops Chickens Cross Reactions Disease Reservoirs DNA, Viral Reverse Transcriptase Polymerase Chain Reaction Rna, Viral Sars Virus Species Specificity Sus Scrofa Vero Cells Virus Replication

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• Source:
  Emerg Infect Dis. 10(2):179-184.
• Document Type:
  Journal Article
• Volume:
  10
• Issue:
  2
• Collection(s):
  Emerging Infectious Diseases
• Main Document Checksum:
  urn:sha256:1ff4765d32e48b1a8c8d7a17ec5620680dc2b46167b6b2bb995f15c45ded157f
• Download URL:
  https://stacks.cdc.gov/view/cdc/14476/cdc_14476_DS1.pdf
• File Type:
  [PDF - 211.92 KB ]