Enzyme-Linked Immunosorbent Assay and Serologic Responses to Pneumocystis jiroveci
Published Date:May 2004
Source:Emerg Infect Dis. 10(5):848-854.
Keywords:AIDS-Related Opportunistic Infections
Enzyme-Linked Immunosorbent Assay
Major Surface Glycoprotein
Funding:AI 25467/AI/NIAID NIH HHS/United States
AI 75319/AI/NIAID NIH HHS/United States
R01 HL64570/HL/NHLBI NIH HHS/United States
Description:Seroepidemiologic studies of Pneumocystis pneumonia (PCP) in humans have been limited by inadequate reagents. We have developed an enzyme-linked immunosorbent assay (ELISA) using three overlapping recombinant fragments of the human Pneumocystis major surface glycoprotein (MsgA, MsgB, and MsgC) for analysis of antibody responses in HIV-positive patients and healthy blood donors. HIV-positive patients had significantly higher antibody levels to all Msg fragments. Furthermore, HIV-positive patients who experienced a previous episode of PCP (PCP-positive) had higher levels of antibodies to MsgC than patients who never had PCP. A significant association was found between ELISA antibody level and reactivity by Western blot in HIV-positive patients, especially those who were PCP-positive. Thus, this ELISA will be useful in studying serum antibody responses to Pneumocystis in different human populations.
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