Enzyme-Linked Immunosorbent Assay and Serologic Responses to Pneumocystis jiroveci

Daly, Kieran R.; Koch, Judy; Levin, Linda; Walzer, Peter D.

doi:10.3201/eid1005.030497

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Enzyme-Linked Immunosorbent Assay and Serologic Responses to Pneumocystis jiroveci

May 2004
By Daly, Kieran R. ; Koch, Judy ; Levin, Linda ; ...
http://dx.doi.org/10.3201/eid1005.030497
Source: Emerg Infect Dis. 10(5):848-854.

[PDF-189.55 KB]

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Details:

Alternative Title:

Emerg Infect Dis
Personal Author:

Daly, Kieran R. ; Koch, Judy ; Levin, Linda ; Walzer, Peter D.

Daly, Kieran R. ; Koch, Judy ; Levin, Linda ; Walzer, Peter D. Less -
Description:

Seroepidemiologic studies of Pneumocystis pneumonia (PCP) in humans have been limited by inadequate reagents. We have developed an enzyme-linked immunosorbent assay (ELISA) using three overlapping recombinant fragments of the human Pneumocystis major surface glycoprotein (MsgA, MsgB, and MsgC) for analysis of antibody responses in HIV-positive patients and healthy blood donors. HIV-positive patients had significantly higher antibody levels to all Msg fragments. Furthermore, HIV-positive patients who experienced a previous episode of PCP (PCP-positive) had higher levels of antibodies to MsgC than patients who never had PCP. A significant association was found between ELISA antibody level and reactivity by Western blot in HIV-positive patients, especially those who were PCP-positive. Thus, this ELISA will be useful in studying serum antibody responses to Pneumocystis in different human populations.
Subjects:

[+]

AIDS-Related Opportunistic Infections Antibodies, Fungal Antigens, Fungal Blood Donors ELISA Enzyme-Linked Immunosorbent Assay Fungal Proteins HIV Infections HIV Patients Humans Infection Major Surface Glycoprotein Membrane Glycoproteins Pneumocystis Pneumonia, Pneumocystis Recombinant Proteins Research Seroepidemiologic Studies Serum Antibodies
Source:

Emerg Infect Dis. 10(5):848-854.
Document Type:

Journal Article
Funding:

AI 25467/AI/NIAID NIH HHS/United States ; AI 75319/AI/NIAID NIH HHS/United States ; R01 HL64570/HL/NHLBI NIH HHS/United States

AI 25467/AI/NIAID NIH HHS/United States ; AI 75319/AI/NIAID NIH HHS/United States ; R01 HL64570/HL/NHLBI NIH HHS/United States Less -
Volume:

10
Issue:

5
Collection(s):

Emerging Infectious Diseases
Main Document Checksum:

[+]

urn:sha256:d9b3a71017543ed281489e18076814179a82289f57c2b0a40c82e44466409f4a
Download URL:

https://stacks.cdc.gov/view/cdc/14410/cdc_14410_DS1.pdf
File Type:

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