Enzyme-Linked Immunosorbent Assay and Serologic Responses to Pneumocystis jiroveci

Details

• Alternative Title:
  Emerg Infect Dis
• Personal Author:
  Daly, Kieran R. ; Koch, Judy ; Levin, Linda ; Walzer, Peter D.
• Description:
  Seroepidemiologic studies of Pneumocystis pneumonia (PCP) in humans have been limited by inadequate reagents. We have developed an enzyme-linked immunosorbent assay (ELISA) using three overlapping recombinant fragments of the human Pneumocystis major surface glycoprotein (MsgA, MsgB, and MsgC) for analysis of antibody responses in HIV-positive patients and healthy blood donors. HIV-positive patients had significantly higher antibody levels to all Msg fragments. Furthermore, HIV-positive patients who experienced a previous episode of PCP (PCP-positive) had higher levels of antibodies to MsgC than patients who never had PCP. A significant association was found between ELISA antibody level and reactivity by Western blot in HIV-positive patients, especially those who were PCP-positive. Thus, this ELISA will be useful in studying serum antibody responses to Pneumocystis in different human populations.
• Subjects:
  • Research
  • Pneumocystis
  • Major surface glycoprotein
  • Infection
  • ELISA
  • HIV patients
  • Serum antibodies
  • AIDS-Related Opportunistic Infections
  • Antibodies, Fungal
  • Antigens, Fungal
  More +
• Source:
  Emerg Infect Dis. 10(5):848-854.
• Document Type:
  Journal Article
• Funding:
  AI 25467/AI/NIAID NIH HHS/United States ; AI 75319/AI/NIAID NIH HHS/United States ; R01 HL64570/HL/NHLBI NIH HHS/United States
• Volume:
  10
• Issue:
  5
• Download URL:
  https://stacks.cdc.gov/view/cdc/14410/cdc_14410_DS1.pdf
• File Type:
  [PDF - 189.55 KB]
• Collection(s):
  Emerging Infectious Diseases
• Main Document Checksum:
  urn:sha256:d9b3a71017543ed281489e18076814179a82289f57c2b0a40c82e44466409f4a