Single Multiplex Polymerase Chain Reaction To Detect Diverse Loci Associated with Diarrheagenic Escherichia coli

Details

• Alternative Title:
  Emerg Infect Dis
• Personal Author:
  López-Saucedo, Catalina ; Cerna, Jorge F. ; Villegas-Sepulveda, Nicolas ; Thompson, Rocío ; Velazquez, F. Raul ; Torres, Javier ; Tarr, Phillip I. ; Estrada-García, Teresa
• Description:
  We developed and tested a single multiplex polymerase chain reaction (PCR) that detects enterotoxigenic, enteropathogenic, enteroinvasive, and Shiga toxin-producing Escherichia coli. This PCR is specific, sensitive, and rapid in detecting target isolates in stool and food. Because of its simplicity, economy, and efficiency, this protocol warrants further evaluation in large, prospective studies of polymicrobial substances.
• Subjects:
  Diagnostic Microbiology Diarrhea Diarrheagenic Escherichia Coli Dispatch DNA Primers Escherichia Coli Escherichia Coli Infections Escherichia Coli Proteins Feces Food Microbiology Food Safety Humans Multiplex PCR Polymerase Chain Reaction Sensitivity And Specificity

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• Source:
  Emerg Infect Dis. 9(1):127-131.
• Document Type:
  Journal Article
• Volume:
  9
• Issue:
  1
• Collection(s):
  Emerging Infectious Diseases
• Main Document Checksum:
  urn:sha256:0de4842d27351b2235519c6b54620af101d7f73881430d83960fa9726888e534
• Download URL:
  https://stacks.cdc.gov/view/cdc/13890/cdc_13890_DS1.pdf
• File Type:
  [PDF - 738.26 KB ]