Prospecting for Zoonotic Pathogens by Using Targeted DNA Enrichment

Enabulele, Egie E.; Le Clec’h, Winka; Roberts, Emma K.; Thompson, Cody W.; McDonough, Molly M.; Ferguson, Adam W.; Bradley, Robert D.; Anderson, Timothy J. C.; Platt, Roy N.

doi:10.3201/eid2908.221818

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Prospecting for Zoonotic Pathogens by Using Targeted DNA Enrichment

8 2023
By Enabulele, Egie E. ; Le Clec’h, Winka ; Roberts, Emma K. ; ...
http://dx.doi.org/10.3201/eid2908.221818
Source: Emerg Infect Dis. 2023; 29(8):1566-1579

[PDF-4.67 MB]

English

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Details:

Alternative Title:

Emerg Infect Dis
Personal Author:

Enabulele, Egie E. ; Le Clec’h, Winka ; Roberts, Emma K. ; Thompson, Cody W. ; McDonough, Molly M. ; ... More +

Enabulele, Egie E. ; Le Clec’h, Winka ; Roberts, Emma K. ; Thompson, Cody W. ; McDonough, Molly M. ; Ferguson, Adam W. ; Bradley, Robert D. ; Anderson, Timothy J. C. ; Platt, Roy N. Less -
Description:

More than 60 zoonoses are linked to small mammals, including some of the most devastating pathogens in human history. Millions of museum-archived tissues are available to understand natural history of those pathogens. Our goal was to maximize the value of museum collections for pathogen-based research by using targeted sequence capture. We generated a probe panel that includes 39,916 80-bp RNA probes targeting 32 pathogen groups, including bacteria, helminths, fungi, and protozoans. Laboratory-generated, mock-control samples showed that we are capable of enriching targeted loci from pathogen DNA 2,882‒6,746-fold. We identified bacterial species in museum-archived samples, including Bartonella, a known human zoonosis. These results showed that probe-based enrichment of pathogens is a highly customizable and efficient method for identifying pathogens from museum-archived tissues.
Subjects:

[+]

Animals Bacteria DNA Fungi Genetic Archive Humans Mammals Natural History Museum Prospecting Prospecting For Zoonotic Pathogens By Using Targeted DNA Enrichment Research Targeted DNA Enrichment United States Zoonoses Zoonotic Pathogens
Source:

Emerg Infect Dis. 2023; 29(8):1566-1579
Pubmed ID:

37486179
Pubmed Central ID:

PMC10370864
Document Type:

Journal Article
Volume:

29
Issue:

8
Collection(s):

Emerging Infectious Diseases
Main Document Checksum:

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urn:sha256:5f5501c9cd2033592b26b3b887ecd845ebea6031e642206f245c588e74624075
Download URL:

https://stacks.cdc.gov/view/cdc/132605/cdc_132605_DS1.pdf
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