Prospecting for Zoonotic Pathogens by Using Targeted DNA Enrichment

Details

• Alternative Title:
  Emerg Infect Dis
• Personal Author:
  Enabulele, Egie E. ; Le Clec’h, Winka ; Roberts, Emma K. ; Thompson, Cody W. ; McDonough, Molly M. ; Ferguson, Adam W. ; Bradley, Robert D. ; Anderson, Timothy J. C. ; Platt, Roy N.
• Description:
  More than 60 zoonoses are linked to small mammals, including some of the most devastating pathogens in human history. Millions of museum-archived tissues are available to understand natural history of those pathogens. Our goal was to maximize the value of museum collections for pathogen-based research by using targeted sequence capture. We generated a probe panel that includes 39,916 80-bp RNA probes targeting 32 pathogen groups, including bacteria, helminths, fungi, and protozoans. Laboratory-generated, mock-control samples showed that we are capable of enriching targeted loci from pathogen DNA 2,882‒6,746-fold. We identified bacterial species in museum-archived samples, including Bartonella, a known human zoonosis. These results showed that probe-based enrichment of pathogens is a highly customizable and efficient method for identifying pathogens from museum-archived tissues.
• Subjects:
  Animals Bacteria DNA Fungi Genetic Archive Humans Mammals Natural History Museum Prospecting Prospecting For Zoonotic Pathogens By Using Targeted DNA Enrichment Research Targeted DNA Enrichment United States Zoonoses Zoonotic Pathogens

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• Source:
  Emerg Infect Dis. 2023; 29(8):1566-1579
• Pubmed ID:
  37486179
• Pubmed Central ID:
  PMC10370864
• Document Type:
  Journal Article
• Volume:
  29
• Issue:
  8
• Collection(s):
  Emerging Infectious Diseases
• Main Document Checksum:
  urn:sha256:5f5501c9cd2033592b26b3b887ecd845ebea6031e642206f245c588e74624075
• Download URL:
  https://stacks.cdc.gov/view/cdc/132605/cdc_132605_DS1.pdf
• File Type:
  [PDF - 4.67 MB ]