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On-column trypsin digestion coupled with LC-MS/MS for quantification of apolipoproteins
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1 06 2017
Source: J Proteomics. 150:258-267 -
Alternative Title:J Proteomics
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Personal Author:
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Description:Significance:
Currently, the translation of apolipoprotein biomarkers to clinical application is impaired because of the high cost of large cohort studies using traditional single-analyte immunoassays. The application of on-line tryptic digestion coupled with LC-MS/MS analysis is an effective way to address this problem. In this work we demonstrate a high throughput, multiplexed, automated proteomics workflow for the simultaneous analysis of multiple proteins.
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Pubmed ID:27667389
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Pubmed Central ID:PMC10071838
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