On-column trypsin digestion coupled with LC-MS/MS for quantification of apolipoproteins

Details

• Alternative Title:
  J Proteomics
• Personal Author:
  Toth, Christopher A ; Kuklenyik, Zsuzsanna ; Jones, Jeffrey I ; Parks, Bryan A ; Gardner, Michael S ; Schieltz, David M ; Rees, Jon C ; Andrews, Michael L ; McWilliams, Lisa G ; Pirkle, James L ; Barr, John R
• Description:
  Significance:
  
  Currently, the translation of apolipoprotein biomarkers to clinical application is impaired because of the high cost of large cohort studies using traditional single-analyte immunoassays. The application of on-line tryptic digestion coupled with LC-MS/MS analysis is an effective way to address this problem. In this work we demonstrate a high throughput, multiplexed, automated proteomics workflow for the simultaneous analysis of multiple proteins.
  
  
• Subjects:
  Apolipoproteins Article Biomarkers Blood Chemical Analysis Blood Proteins Chromatography, Liquid Feasibility Studies Humans Proteolysis Proteomics Reproducibility Of Results Tandem Mass Spectrometry Trypsin

  More +
• Source:
  J Proteomics. 150:258-267
• Pubmed ID:
  27667389
• Pubmed Central ID:
  PMC10071838
• Document Type:
  Journal Article
• Funding:
  CC999999/ImCDC/Intramural CDC HHSUnited States/
• Volume:
  150
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:fdc4daca05b31d3b0bcc59962519a86637c898be5b0b256ef54cbf92d56820b4
• Download URL:
  https://stacks.cdc.gov/view/cdc/126581/cdc_126581_DS1.pdf
• File Type:
  [PDF - 1.85 MB ]