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Multi-Site Proficiency Testing for Validation and Standardization of Assays to Detect Specific Pathogen Free Viruses, Coronaviruses, and Other Agents in Nonhuman Primates
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8 2022
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Source: J Med Primatol. 51(4):234-245
[PDF-623.51 KB]
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Alternative Title:J Med Primatol
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Description:In efforts to increase rigor and reproducibility, the USA National Primate Research Centers (NPRCs) have focused on qualification of reagents, cross-laboratory validations, and proficiency testing for methods to detect infectious agents and accompanying immune responses in nonhuman primates. The pathogen detection working group, comprised of laboratory scientists, colony managers, and leaders from the NPRCs, has championed the effort to produce testing that is reliable and consistent across laboratories. Through multi-year efforts with shared proficiency samples, testing percent agreement has increased from as low as 67.1% for SRV testing in 2010 to 92.1% in 2019. The 2019 average agreement for the four basic SPF agents improved to >96% (86.5% BV, 98.9 SIV, 92.1 SRV, and 97.0 STLV). As new pathogens such as SARS coronavirus type 2 emerge, these steps can now be quickly replicated to develop and implement new assays that ensure rigor, reproducibly, and quality for NHP pathogen detection.
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Pubmed ID:35426147
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Pubmed Central ID:PMC9851150
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Volume:51
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Issue:4
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