Rapid In Vivo Detection of Rat Spinal Cord Injury with Double Diffusion Encoded Magnetic Resonance Spectroscopy

Details

• Alternative Title:
  Magn Reson Med
• Personal Author:
  Skinner, Nathan P ; Kurpad, Shekar N ; Schmit, Brian D ; Muftuler, L. Tugan ; Budde, Matthew D
• Description:
  Purpose ; Diffusion weighted imaging is a common experimental tool for evaluating spinal cord injury (SCI), yet suffers from complications decreasing its clinical effectiveness. The most commonly used technique, Diffusion Tensor Imaging (DTI), is often confounded by effects of edema accompanying acute SCI, limiting its sensitivity to the important functional status marker of axonal integrity. The purpose of this study is to introduce a novel diffusion acquisition method with the goal of overcoming these limitations. ; Methods ; A Double Diffusion Encoding (DDE) pulse sequence was implemented with a diffusion-weighted filter orthogonal to the spinal cord for suppressing non-neural signals prior to diffusion weighting parallel to the cord. A Point-RESolved Spectroscopy readout (DDE-PRESS) was used for improved sensitivity and compared with DTI in a rat model of SCI with varying injury severities. ; Results ; The DDE-PRESS parameter, restricted fraction, showed a strong relationship with injury severity (p<0.001, R2=0.67). Whereas whole-cord averaged DTI parameter values exhibited only minor injury relationships, a weighted ROI-based DTI analysis improved sensitivity to injury (p<0.001, R2=0.66). ; Conclusion ; In a rat model of SCI, DDE-PRESS demonstrated high sensitivity to injury with substantial decreases in acquisition time and data processing. This method shows promise for application in rapid evaluation of SCI severity.
• Subjects:
  • Algorithms
  • Animals
  • Diffusion Tensor Imaging
  • Female
  • Image Enhancement
  • Image Interpretation, Computer-Assisted
  • Magnetic Resonance Spectroscopy
  • Rats
  • Rats, Sprague-Dawley
  • Reproducibility of Results
  More +
• Source:
  Magn Reson Med. 77(4):1639-1649
• Pubmed ID:
  27080726
• Pubmed Central ID:
  PMC5285487
• Document Type:
  Journal Article
• Funding:
  TL1 TR001437/TR/NCATS NIH HHSUnited States/ ; I01 RX001497/RX/RRD VAUnited States/ ; U01 CE002944/CE/NCIPC CDC HHSUnited States/ ; T32 GM080202/GM/NIGMS NIH HHSUnited States/ ; UL1 TR001436/TR/NCATS NIH HHSUnited States/ ; U01 NS088034/NS/NINDS NIH HHSUnited States/
• Volume:
  77
• Issue:
  4
• Download URL:
  https://stacks.cdc.gov/view/cdc/119156/cdc_119156_DS1.pdf
• File Type:
  [PDF - 1.97 MB]
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:e631f798afe41f10bfcea82f389ca8ab0bbd74190dc449ce0979fe0be9c726f1