Details:

Alternative Title:
Adv Healthc Mater
Publisher's site:
http://dx.doi.org/10.1002/adhm.202102539
Personal Author:
Kalelkar, Pranav P. ; Moustafa, Dina A. ; Riddick, Milan ; Goldberg, Joanna B. ; McCarty, Nael A. ; ... More +
Kalelkar, Pranav P. ; Moustafa, Dina A. ; Riddick, Milan ; Goldberg, Joanna B. ; McCarty, Nael A. ; García, Andrés J. Less -
Description:
Lung infections caused by Gram-positive Staphylococcus aureus (S. aureus) and coinfections caused by S. aureus and Gram-negative Pseudomonas aeruginosa (P. aeruginosa) are challenging to treat, especially with the rise in the number of antibiotic-resistant strains of these pathogens. Bacteriophage (phage) are bacteria-specific viruses that can infect and lyse bacteria, providing a potentially effective therapy for bacterial infections. However, the development of bacteriophage therapy is impeded by limited suitable biomaterials that can facilitate effective delivery of phage to the lung. Here, the ability of porous microparticles engineered from poly(lactic-co-glycolic acid) (PLGA), a biodegradable polyester, to effectively deliver phage to the lung, is demonstrated. The phage-loaded microparticles (phage-MPs) display potent antimicrobial efficacy against various strains of S. aureus in vitro and in vivo, and arrest the growth of a clinical isolate of S. aureus in the presence of sputum supernatant obtained from cystic fibrosis patients. Moreover, phage-MPs efficiently mitigate in vitro cocultures of S. aureus and P. aeruginosa and display excellent cytocompatibility with human lung epithelial cells. Therefore, phage-MPs represents a promising therapy to treat bacterial lung infection.
Subjects:
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Anti-Bacterial Agents Antimicrobial Biomaterials Article Bacterial Infections Bacteriophages Coculture Techniques Cystic Fibrosis Glycols Humans Phage Therapy Poly(lactic-co-glycolic Acid) Polyesters Polylactic Acid-Polyglycolic Acid Copolymer Pseudomonas Aeruginosa Staphylococcal Infections Staphylococcus Aureus
Source:
Adv Healthc Mater. 11(10):e2102539
Pubmed ID:
34957709
Pubmed Central ID:
PMC9117426
Document Type:
Journal Article
Funding:
Parker H. Petit Institute for Bioengineering and Bioscience at the Georgia Institute of Technology/ ; Airways Disease Research, Children's Healthcare of Atlanta and Emory University/ ; Cystic Fibrosis Biospecimen Repository at the Children's Healthcare of Atlanta and Emory University CF Discovery Core/ ; NIH R01 AR062920/NH/NIH HHSUnited States/ ; R01 AR062920/AR/NIAMS NIH HHSUnited States/ ; ... More +
Parker H. Petit Institute for Bioengineering and Bioscience at the Georgia Institute of Technology/ ; Airways Disease Research, Children's Healthcare of Atlanta and Emory University/ ; Cystic Fibrosis Biospecimen Repository at the Children's Healthcare of Atlanta and Emory University CF Discovery Core/ ; NIH R01 AR062920/NH/NIH HHSUnited States/ ; R01 AR062920/AR/NIAMS NIH HHSUnited States/ ; Animal Models Core of the Center/ ; CFF GARCIA17G0/Cystic Fibrosis Foundation/ ; CC/CDC HHSUnited States/ Less -
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:c82a467560a4a71a3c4e4f3a98b75047d1bb1f1d67ad9e294a7b2300a09b073f
Download URL:
https://stacks.cdc.gov/view/cdc/117638/cdc_117638_DS1.pdf
File Type:

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