Whole-Genome Sequencing Reveals the Presence of the blaCTX-M-65 Gene in Extended-Spectrum β-Lactamase-Producing and Multi-Drug-Resistant Clones of Salmonella Serovar Infantis Isolated from Broiler Chicken Environments in the Galapagos Islands

Details

• Alternative Title:
  Antibiotics (Basel)
• Personal Author:
  Burnett, Elton ; Ishida, Maria ; de Janon, Sofia ; Naushad, Sohail ; Duceppe, Marc-Olivier ; Gao, Ruimin ; Jardim, Armando ; Chen, Jessica C. ; Tagg, Kaitlin A. ; Ogunremi, Dele ; Vinueza-Burgos, Christian
• Description:
  Infantis, a common contaminant of poultry products, is known to harbor mobile genetic elements that confer multi-drug resistance (MDR) and have been detected in many continents. Here, we report four MDR |. | strains recovered from poultry house environments in Santa Cruz Island of the Galapagos showing extended-spectrum β-lactamase (ESBL) resistance and reduced fluoroquinolone susceptibility. Whole-genome sequencing (WGS) revealed the presence of the ESBL-conferring | gene in an IncFIB-like plasmid in three |. Infantis isolates. Multi-locus sequence typing (MLST) and single nucleotide variant/polymorphism (SNP) SNVPhyl analysis showed that the |. Infantis isolates belong to sequence type ST32, likely share a common ancestor, and are closely related (1-3 SNP difference) to |-containing clinical and veterinary |. Infantis isolates from the United States and Latin America. Furthermore, phylogenetic analysis of SNPs following core-genome alignment (i.e., ParSNP) inferred close relatedness between the |. Infantis isolates from Galapagos and the United States. Prophage typing confirmed the close relationship among the Galapagos |. Infantis and was useful in distinguishing them from the United States isolates. This is the first report of MDR |-containing |. Infantis in the Galapagos Islands and highlights the need for increased monitoring and surveillance programs to determine prevalence, sources, and reservoirs of MDR pathogens.
• Subjects:
  Article Extended-spectrum β-lactamase Galapagos Multi-drug Resistance Salmonella Infantis
• Source:
  Antibiotics (Basel). 2021; 10(3)
• Pubmed ID:
  33807748
• Pubmed Central ID:
  PMC8000398
• Document Type:
  Journal Article
• Volume:
  10
• Issue:
  3
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:1c125b43ebbe51bcfe9359e6487d5d60e4053b3844bca974ada09a5c19af7af4
• Download URL:
  https://stacks.cdc.gov/view/cdc/111613/cdc_111613_DS1.pdf
• File Type:
  [PDF - 1.31 MB ]