Determination of Anthocyanins in Cranberry Fruit and Cranberry Fruit Products by High-Performance Liquid Chromatography with Ultraviolet Detection: Single-Laboratory Validation

Details

• Alternative Title:
  J AOAC Int
• Personal Author:
  Brown, Paula N. ; Shipley, Paul R.
• Description:
  A single-laboratory validation study was conducted on an HPLC method for the detection and quantification of cyanidin-3-O-galactoside (C3Ga), cyanidin-3-O-glucoside (C3GI), cyanidin-3-O-arabinoside (C3Ar), peonidin-3-O-galactoside (P3Ga), and peonidin-3-O-arabinoside (P3Ar) in cranberry fruit (Vaccinium macrocarpon Aiton) raw material and finished products. An extraction procedure using a combination of sonication and shaking with acidified methanol was optimized for all five anthocyanins in freeze-dried cranberry fruit and finished products (commercial extract powder, juice, and juice cocktail). Final extract solutions were analyzed by HPLC using a C18 RP column. Calibration curves for all anthocyanin concentrations had correlation coefficients (r2) of > or = 99.8%. The method detection limits for C3Ga, C3Gl, C3Ar, P3Ga, and P3Ar were estimated to be 0.018, 0.016, 0.006, 0.013, and 0.011 microg/mL, respectively. Separation was achieved with a chromatographic run time of 35 min using a binary mobile phase with gradient elution. Quantitative determination performed in triplicate on four test materials on each of 3 days (n = 12) resulted in RSD(r) from 1.77 to 3.31%. Analytical range, as defined by the calibration curves, was 0.57-36.53 microg/mL for C3Ga, 0.15-9.83 microg/mL for C3GI, 0.28-17.67 microg/mL for C3Ar, 1.01-64.71 microg/mL for P3Ga, and 0.42-27.14 microg/mL for P3Ar. For solid materials prepared by the described method, this translates to 0.06-3.65 mglg for C3Ga, 0.02-0.98 mg/g for C3Gl, 0.03-1.77 mg/g for C3Ar, 0.10-6.47 mg/g for P3Ga, and 0.04-2.71 mg/g for P3Ar.
• Subjects:
  Anthocyanins Article Beverages Chromatography, High Pressure Liquid Fruit Plant Extracts Ultraviolet Rays Vaccinium Macrocarpon
• Source:
  J AOAC Int. 94(2):459-466
• Pubmed ID:
  21563679
• Pubmed Central ID:
  PMC3302353
• Document Type:
  Journal Article
• Funding:
  223-01-2464/CD/ODCDC CDC HHSUnited States/
• Volume:
  94
• Issue:
  2
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:3f66a2d6d043bd2494b62fe7b3b903925dd3f42574c1940492c6f17adbe0465a
• Download URL:
  https://stacks.cdc.gov/view/cdc/110928/cdc_110928_DS1.pdf
• File Type:
  [PDF - 435.07 KB ]