One Year Survey of Human Rotavirus Strains Suggests the Emergence of Genotype G12 in Cameroon

Details

• Alternative Title:
  J Med Virol
• Personal Author:
  Ndze, Valentine N. ; Papp, Hajnalka ; Achidi, Eric A. ; Gonsu, Kamga H. ; László, Brigitta ; Farkas, Szilvia ; Kisfali, Péter ; Melegh, Béla ; Esona, Mathew D. ; Bowen, Michael D. ; Bányai, K. ; Gentsch, Jon R. ; Odama, Abena M.T.
  More +
• Description:
  In this study the emergence of rotavirus A genotype G12 in children <5 years of age is reported from Cameroon during 2010/2011. A total of 135 human stool samples were P and G genotyped by reverse transcriptase PCR. Six different rotavirus VP7 genotypes were detected, including G1, G2, G3, G8, G9, and G12 in combinations with P4, P6 and P8 VP4 genotypes. Genotype G12 predominated in combination with P8 (54.1%) and P6 (10.4%) genotypes followed by G1P6 (8.2%), G3P6 (6.7%), G2P4 (5.9%), G8P6 (3.7%), G2P6 (0.7%), G3P8 (0.7%), and G9P8 (0.7%). Genotype P6 strains in combination with various G-types represented a substantial proportion (N=44, 32.6%) of the genotyped strains. Partially typed strains included G12PNT (2.2%); G3PNT (0.7%); G(NT)P6 (1.5%); and G(NT)P8 (0.7%). Mixed infections were found in five specimens (3.7%) in several combinations including G1+ G12P6, G2+ G3P6 + P8, G3+ G8P6, G3 + G12P6 + P8, and G12P6 +P8. The approximately 10% relative frequency of G12P6 strains detected in this study suggests that this strain is emerging in Cameroon and should be monitored carefully as rotavirus vaccine is implemented in this country, as it shares neither G- nor P-type specificity with strains in the RotaTeq® and Rotarix® vaccines. These findings are consistent with other recent reports of the global spread and increasing epidemiologic importance of G12 and P6 strains.
• Subjects:
  • Child, Preschool
  • Coinfection
  • Feces
  • Genotype
  • Humans
  • Infant
  • Molecular Epidemiology
  • RNA, Viral
  • Reverse Transcriptase Polymerase Chain Reaction
  • Rotavirus
  More +
• Source:
  J Med Virol. 85(8):1485-1490
• Pubmed ID:
  23765785
• Pubmed Central ID:
  PMC8167840
• Document Type:
  Journal Article
• Funding:
  CC999999/ImCDC/Intramural CDC HHS/United States
• Place as Subject:
  Cameroon
• Volume:
  85
• Issue:
  8
• Download URL:
  https://stacks.cdc.gov/view/cdc/106786/cdc_106786_DS1.pdf
• File Type:
  [PDF - 471.82 KB]
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:59c158b93bd27416534f504a2ce0e7b7d63edd77458b71affaac19479be526d6