Engineered NS1 for Sensitive, Specific Zika Virus Diagnosis from Patient Serology

Details

• Alternative Title:
  Emerg Infect Dis
• Personal Author:
  Yap, Thai Leong ; Hong, Shin Yee ; Soh, Jun Hui ; Ravichandraprabhu, Lekha ; Lim, Vanessa W.X. ; Chan, Hsi-Min ; Ong, Tommy Z.X. ; Chua, Ying Ping ; Koh, Shi En ; Wang, Huajing ; Leo, Yee Sin ; Ying, Jackie Y. ; Sun, William
• Description:
  Dengue virus (DENV) and Zika virus (ZIKV) belong to the Flaviviridae family of viruses spread by Aedes aegypti mosquitoes in tropical and subtropical areas. Accurate diagnostic tests to differentiate the 2 infections are necessary for patient management and disease control. Using characterized ZIKV and DENV patient plasma in a blind manner, we validated an ELISA and a rapid immunochromatographic test for ZIKV detection. We engineered the ZIKV nonstructural protein 1 (NS1) for sensitive serologic detection with low cross reactivity against dengue and developed monoclonal antibodies specific for the ZIKV NS1 antigen. As expected, the serologic assays performed better with convalescent than acute plasma samples; the sensitivity ranged from 71% to 88%, depending on the performance of individual tests (IgM/IgG/NS1). Although serologic tests were generally less sensitive with acute samples, our ZIKV NS1 antibodies were able to complement the serologic tests to achieve greater sensitivity for detecting early infections.
• Subjects:
  Dengue Diagnostics Engineered Ns1 From Patient Serology For Sensitive, Specific Zika Virus Diagnosis Enzyme-Linked Immunosorbent Assay Flavivirus Immunochromatography Immunoglobulin Microcephaly Monoclonal Antibody Research Serologic Test Viruses Zika Zika Virus

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• Source:
  Emerg Infect Dis. 27(5):1427-1437
• Pubmed ID:
  33900180
• Pubmed Central ID:
  PMC8084482
• Document Type:
  Journal Article
• Volume:
  27
• Issue:
  5
• Collection(s):
  Emerging Infectious Diseases
• Main Document Checksum:
  urn:sha256:be1e3c1745f3a0b5b8cc8e3b83387f8661df519182c0194cf74340a28f8416d2
• Download URL:
  https://stacks.cdc.gov/view/cdc/106003/cdc_106003_DS1.pdf
• File Type:
  [PDF - 1.92 MB ]