A single-dose live-attenuated Zika virus vaccine with controlled infection rounds that protects against vertical transmission

Details

• Alternative Title:
  Cell Host Microbe
• Personal Author:
  Xie, Xuping ; Kum, Dieudonné B. ; Xia, Hongjie ; Luo, Huanle ; Shan, Chao ; Zou, Jing ; Muruato, Antonio E. ; Medeiros, Daniele B. A. ; Nunes, Bruno T. D. ; Dallmeier, Kai ; Rossi, Shannan L. ; Weaver, Scott C. ; Neyts, Johan ; Wang, Tian ; Vasconcelos, Pedro F. C. ; Shi, Pei-Yong
• Description:
  Zika virus (ZIKV) infection of the mother during pregnancy causes devastating Zika congenital syndrome in the offspring. A ZIKV vaccine with optimal safety and immunogenicity for use in pregnant women is critically needed. Toward this goal, we have developed a single-dose live-attenuated vaccine candidate that infects cells with controlled, limited infection rounds. The vaccine contains a 9-amino-acid deletion in the viral capsid protein and replicates to titers of > 10| focus-forming units (FFU)/mL in cells expressing the full-length capsid protein. Immunization of A129 mice with one dose (10| FFU) did not produce viremia, but elicited protective immunity that completely prevented viremia, morbidity, and mortality after challenge with an epidemic ZIKV strain (10| PFU). A single-dose vaccination also fully prevented infection of pregnant mice and maternal-to-fetal transmission. Intracranial injection of the vaccine (10| FFU) to 1-day-old mice did not cause any disease or death, underscoring the safety of this vaccine candidate.
• Subjects:
  A549 Cells Animals Capsid Proteins Cell Line Chlorocebus Aethiops Cricetinae Female Humans Infectious Disease Transmission, Vertical Male Mice Mice, Inbred Strains Mice, Knockout Pregnancy Vaccines, Attenuated Vero Cells Viral Vaccines Zika Virus Zika Virus Infection

  More +
• Keywords:
  Flavivirus Capsid Vaccine Zika Virus
• Source:
  Cell Host Microbe. 24(4):487-499.e5
• Pubmed ID:
  30308155
• Pubmed Central ID:
  PMC6188708
• Document Type:
  Journal Article
• Funding:
  R01 AI127744/AI/NIAID NIH HHSUnited States/ ; R24 AI120942/AI/NIAID NIH HHSUnited States/ ; U01 CK000512/CK/NCEZID CDC HHSUnited States/ ; U01CK000512/ACL/ACL HHSUnited States/
• Volume:
  24
• Issue:
  4
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:7c489b3b64b1124eb3fac9855c65657afba4e4bfb74894308faad3e61e2db7f5
• Download URL:
  https://stacks.cdc.gov/view/cdc/82045/cdc_82045_DS1.pdf
• File Type:
  [PDF - 2.67 MB ]